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A large number of polyclonal antibodies from other suppliers will soon be unavailable, potentially delaying your research. However, we have many great alternatives.
The top-cited anti-ERK1 antibody, for example, will be discontinued by another antibody supplier by 2017. In addition to its limited supply, this antibody cross-reacts with other proteins (figure 1), making your data harder to accurately interpret.
Figure 1. Western blot results for another supplier's top-cited anti-ERK1 antibody. Predicted band size: 43 kDa. Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg). Lanes 2, 4 and 6: ERK1 knockout HAP1 cell lysate (20 µg). Lanes 1 and 2: green signal from top-cited anti-ERK1 antibody. Lanes 3 and 4: red signal from loading control (ab8245). Lanes 5 and 6: merged (red and green) signal.
As a replacement for this, we have an anti-ERK1 RabMAb® rabbit monoclonal that has been knockout validated with CRISPR technology. Not only does offer highly specific binding (figure 2), but provides you with consistent batch-to-batch results and a guaranteed security of supply.
Figure 2. Left: western blot results from our anti-ERK1 antibody (ab32537). Predicted band size: 43 kDa. Lanes 1, 3 and 5: wild-type HAP1 cell lysate (20 µg). Lanes 2, 4 and 6: ERK1 knockout HAP1 cell lysate (20 µg). Lanes 1 and 2: green signal from target (ab32537) observed at 42 kDa. Lanes 3 and 4: red signal from loading control (ab8226) observed at 42 kDa. Lanes 5 and 6: merged (red and green) signal. Right: immunocytochemistry data from our anti-ERK1 antibody (ab32537) in wild-type HAP1 cells (top panel) and ERK1 knockout HAP1 cells (bottom panel). DNA labeled in blue with DAPI.
If you want to ensure the supply of your antibodies and accuracy of your research, we can help you find replacement options for those