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Our Abpromise guarantee covers the use of ab2777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 3 - 5 µg/ml.
Immunohistochemical staining of PrP(Sc) in infected sheep brain results in intense staining of the spongiform lesions. In immunohistochemical procedures this antibody detects only the PrP(Sc) and not PrP(C).
|WB||Use a concentration of 3 - 5 µg/ml. Can be blocked with Prion protein PrP peptide (ab4908).
By Western blot, this antibody detects a 33-35 kDa protein from normal animals and a 27-30 kDa protein which represents PrP in brain protease treated tissue extracts from infected animals.
|Flow Cyt||Use 0.1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.