Recombinant Anti-PKN3 (phospho T718) + PRK1 (phospho T774) + PKN2 (phospho T816) antibody [EPR5671] (ab124709)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5671] to PKN3 (phospho T718) + PRK1 (phospho T774) + PKN2 (phospho T816)
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PKN3 (phospho T718) + PRK1 (phospho T774) + PKN2 (phospho T816) antibody [EPR5671] -
Description
Rabbit monoclonal [EPR5671] to PKN3 (phospho T718) + PRK1 (phospho T774) + PKN2 (phospho T816) -
Host species
Rabbit -
Specificity
This antibody only detects PKN3 phosphorylated at Threonine 718, PKN2 phosphorylated at Threonine 816, and PKN1 phosphorylated at Threonine 774. -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. -
Positive control
- HeLa cell lysates and Hek 293 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5671 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 104, 112, 99 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 104, 112, 99 kDa. |
Target
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Cellular localization
PKN3: Nucleus. Cytoplasm > perinuclear region. Nuclear and perinuclear Golgi region. PRK1: Cytoplasmic PKN2: Cytoplasm. -
Database links
- Entrez Gene: 29941 Human
- Entrez Gene: 5585 Human
- Entrez Gene: 5586 Human
- Entrez Gene: 109333 Mouse
- Entrez Gene: 263803 Mouse
- Entrez Gene: 320795 Mouse
- Entrez Gene: 207122 Rat
- Entrez Gene: 296619 Rat
see all
Images
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All lanes : Anti-PKN3 (phospho T718) + PRK1 (phospho T774) + PKN2 (phospho T816) antibody [EPR5671] (ab124709) at 1/1000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate treated with Okadaic acid and Calyculin A.
Lysates/proteins at 10 µg per lane.
Predicted band size: 104, 112, 99 kDa
Bottom blot was probed with an anti-PNK2 antibody. -
ab124709, at 1/10, staining PKN1 (phospho T774) + PKN2 (phospho T816) + PKN3 (phospho T718) in permeabilized Hek 293 cells by intracellular flow cytometry. Green: untreated cells Red: Okadaic Acid + Calyculin A-treated cells Blue: untreated cells preincubated with the immunizing phosphopeptides Orange: Okadaic Acid + Calyculin A-treated cells preincubated with the immunizing phosphopeptides.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab124709 has been referenced in 2 publications.
- Bai Y et al. Phosphorylation-mediated PI3K-Art signalling pathway as a therapeutic mechanism in the hydrogen-induced alleviation of brain injury in septic mice. J Cell Mol Med 26:5713-5727 (2022). PubMed: 36308410
- Uehara S et al. Protein kinase N3 promotes bone resorption by osteoclasts in response to Wnt5a-Ror2 signaling. Sci Signal 10:N/A (2017). PubMed: 28851822