Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709)

Overview

  • Product nameAnti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671]
  • Description
    Rabbit monoclonal [EPR5671] to PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)
  • SpecificityThis antibody only detects PKN3 phosphorylated at Threonine 718, PKN2 phosphorylated at Threonine 816, and PKN1 phosphorylated at Threonine 774.
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues surrounding Threonine 718 of Human PKN3 (Q6P5Z2), Threonine 816 of Human PKN2 (Q16513), and Threonine 774 of Human PKN1 (Q16512) proteins.

  • Positive control
    • HeLa cell lysates and Hek 293 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 104, 112, 99 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC/IF,IHC-P or IP.
  • Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] images

    • All lanes : Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709) at 1/1000 dilution

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate treated with Okadaic acid and Calyculin A.

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 104, 112, 99 kDa
    • ab124709, at 1/10, staining PKN1 (phospho T774) + PKN2 (phospho T816) + PKN3 (phospho T718) in permeabilized Hek 293 cells by Flow cytometry.
      Green: untreated cells
      Red: Okadaic Acid + Calyculin A-treated cells
      Blue: untreated cells preincubated with the immunizing phosphopeptides
      Orange: Okadaic Acid + Calyculin A-treated cells preincubated with the immunizing phosphopeptides .

    References for Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709)

    ab124709 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124709.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"