Recombinant
RabMAb

Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709)

Overview

  • Product name
    Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671]
  • Description
    Rabbit monoclonal [EPR5671] to PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)
  • Host species
    Rabbit
  • Specificity
    This antibody only detects PKN3 phosphorylated at Threonine 718, PKN2 phosphorylated at Threonine 816, and PKN1 phosphorylated at Threonine 774.
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues surrounding Threonine 718 of Human PKN3 (Q6P5Z2), Threonine 816 of Human PKN2 (Q16513), and Threonine 774 of Human PKN1 (Q16512) proteins.

  • Positive control
    • HeLa cell lysates and Hek 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 104, 112, 99 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Images

    • All lanes : Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709) at 1/1000 dilution

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate treated with Okadaic acid and Calyculin A.

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 104, 112, 99 kDa



      Bottom blot was probed with an anti-PNK2 antibody.
    • ab124709, at 1/10, staining PKN1 (phospho T774) + PKN2 (phospho T816) + PKN3 (phospho T718) in permeabilized Hek 293 cells by Flow cytometry.
      Green: untreated cells
      Red: Okadaic Acid + Calyculin A-treated cells
      Blue: untreated cells preincubated with the immunizing phosphopeptides
      Orange: Okadaic Acid + Calyculin A-treated cells preincubated with the immunizing phosphopeptides .

    References

    ab124709 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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