Overview

  • Product name
  • Description
    Rabbit polyclonal to PRKCDBP
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the center region of human PRKCDBP

  • Positive control
    • A2058 cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/1000.
    WB: 1/50 - 1/100. Predicted molecular weight: 28 kDa.
    Bands at 40-45 kDa may also occur due to 2 possible prenylation sites

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Plays a role in the regulation of the circadian clock. Modulates the period length and phase of circadian gene expression and also regulates expression and interaction of the core clock components PER1/2 and CRY1/2. Seems to have an immune potentiation function.
    • Tissue specificity
      Strongly expressed in mammary and epithelial cells.
    • Sequence similarities
      Belongs to the PTRF/SDPR family.
    • Post-translational
      modifications
      In vitro, phosphorylated by PRKCD.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cavin 3 antibody
      • Cavin-3 antibody
      • CAVIN3 antibody
      • hSRBC antibody
      • MGC20400 antibody
      • PRDBP_HUMAN antibody
      • Prkcdbp antibody
      • Protein kinase C delta binding protein antibody
      • Protein kinase C delta-binding protein antibody
      • Sdr related gene product that binds to c kinase antibody
      • Serum deprivation response factor related gene product that binds to C kinase antibody
      • Serum deprivation response factor-related gene product that binds to C-kinase antibody
      • SRBC antibody
      see all

    Anti-PRKCDBP antibody images

    • Anti-PRKCDBP antibody (ab76676) at 1/50 dilution + A2058 cell lysate at 35 µg

      Predicted band size : 28 kDa
      Observed band size : 29 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-PRKCDBP antibody (ab76676)

    This product has been referenced in:
    • Sathish V  et al. Inflammation, caveolae and CD38-mediated calcium regulation in human airway smooth muscle. Biochim Biophys Acta 1843:346-51 (2014). Read more (PubMed: 24275509) »

    See 1 Publication for this product

    Product Wall

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (Lung)
    Specification
    Lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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