Overview

  • Product name
  • Description
    Goat polyclonal to PRKCDBP
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to internal amino acids 178-190 (

    C-RRTGLQKVQSLR

    ) of Human PRKCDBP (NP_659477.2).

  • Positive control
    • Human Adipose and NIH3T3 cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 28 kDa.

Target

  • Function
    Plays a role in the regulation of the circadian clock. Modulates the period length and phase of circadian gene expression and also regulates expression and interaction of the core clock components PER1/2 and CRY1/2. Seems to have an immune potentiation function.
  • Tissue specificity
    Strongly expressed in mammary and epithelial cells.
  • Sequence similarities
    Belongs to the PTRF/SDPR family.
  • Post-translational
    modifications
    In vitro, phosphorylated by PRKCD.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cavin 3 antibody
    • Cavin-3 antibody
    • CAVIN3 antibody
    • hSRBC antibody
    • MGC20400 antibody
    • PRDBP_HUMAN antibody
    • Prkcdbp antibody
    • Protein kinase C delta binding protein antibody
    • Protein kinase C delta-binding protein antibody
    • Sdr related gene product that binds to c kinase antibody
    • Serum deprivation response factor related gene product that binds to C kinase antibody
    • Serum deprivation response factor-related gene product that binds to C-kinase antibody
    • SRBC antibody
    see all

Images

  • All lanes : Anti-PRKCDBP antibody (ab99427) at 0.3 µg/ml

    Lane 1 : Mouse ovary lysates
    Lane 2 : Rat uterus lysates

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 28 kDa
  • Anti-PRKCDBP antibody (ab99427) at 0.1 µg/ml + Adipose cell lysate (in RIPA buffer) at 35 µg

    Predicted band size : 28 kDa
    Primary incubation was 1 hour.

    Detected by chemiluminescence.

References

This product has been referenced in:
  • Mohan J  et al. Cavin3 interacts with cavin1 and caveolin1 to increase surface dynamics of caveolae. J Cell Sci 128:979-91 (2015). Read more (PubMed: 25588833) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (adipose)
Loading amount
10 µg
Specification
adipose
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

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Verified customer

Submitted May 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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