The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
1/2000 - 1/10000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
RelevancePRKRIR is upstream regulator of interferon induced serine/threonine protein kinase R (PKR). It may block the PKR inhibitory function of P58IPK, resulting in the restoration of kinase activity and suppression of cell growth.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling PRKRIR with ab70333 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
Western blot - PRKRIR antibody (ab70333)
All lanes : Anti-PRKRIR antibody (ab70333) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Lane 4 : Whole cell lysate from mouse NIH3T3 cells at 50 µg
Predicted band size : 88 kDa Observed band size : 88 kDa
Immunoprecipitation - PRKRIR antibody (ab70333)
HeLa whole cell lysate, (1 mg for IP; 1/4 of IP reaction loaded) with ab70333 at 3 µg/mg lysate for IP, and 1µg/ml for subsequent WB.
Detection: Chemiluminescence with an exposure time of 30 seconds.
References for Anti-PRKRIR antibody (ab70333)
has not yet been referenced specifically in any publications.
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