Overview

  • Product name
    Anti-PRKRIR antibody
  • Description
    Rabbit polyclonal to PRKRIR
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (Human) corresponding to a region between residues 150 and 200 of human PRKRIR (NP_004696).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
IP Use a concentration of 1 - 4 µg/ml.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    PRKRIR is upstream regulator of interferon induced serine/threonine protein kinase R (PKR). It may block the PKR inhibitory function of P58IPK, resulting in the restoration of kinase activity and suppression of cell growth.
  • Cellular localization
    Nucleus
  • Database links
  • Alternative names
    • 52 kDa repressor of the inhibitor of the protein kinase antibody
    • 58 kDa interferon induced protein kinase interacting protein antibody
    • DAP4 antibody
    • Death associated protein 4 antibody
    • Inhibitor of protein kinase PKR antibody
    • Interferon inducible double stranded RNA dependent inhibitor antibody
    • MGC102750 antibody
    • P52rIPK antibody
    • P58 repressor antibody
    • p58IPK interacting protein antibody
    • THAP domain containing protein 0 antibody
    • THAP0 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling PRKRIR with ab70334 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PRKRIR antibody (ab70334) at 0.04 µg/ml

    Lane 1 : whole cell lysate of HeLa cells at 50 µg
    Lane 2 : whole cell lysate of HeLa cells at 15 µg
    Lane 3 : whole cell lysate of HeLa cells at 5 µg
    Lane 4 : whole cell lysate of NIH3T3 cells at 50 µg


    Predicted band size : 88 kDa
    Observed band size : 88 kDa
  • IP/WB - 1 mg whole cell lysate of HeLa cells used, and 1/4 of the reaction loaded.
    Lane 1: ab70333 used at 3µg/ml lysate for IP.
    Lane 2: ab70334 used at 3µg/ml lysate for IP.
    Lane 3: IgG control.
    ab70334 used at at 1µg/ml for the subsequent WB for all lanes.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab70334 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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