Overview

  • Product name
  • Description
    Rabbit polyclonal to PRKX
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 44-309 of Human PRKX (Uniprot ID: P51817).

  • Positive control
    • 293T, A431 and Mouse brain whole cell lysates, H3347 xenograft tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8.

Target

  • Tissue specificity
    High levels in adult and fetal brain, kidney and lung; low levels in adult placenta, heart, liver, skeletal muscle, pancreas and fetal liver.
  • Involvement in disease
    Note=A chromosomal aberration involving PRKX is a cause of sex reversal disorder. Translocation t(X;Y)(p22;p11) with PRKY. Chromosomal translocations proximal to PRKY account for about 30% of the cases of sex reversal disorder in XX males and XY females.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • PKX1 antibody
    • PRKX antibody
    • PRKX_HUMAN antibody
    • Protein kinase PKX1 antibody
    • Serine/threonine-protein kinase PRKX antibody
    see all

Images

  • Anti-PRKX antibody (ab154739) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 41 kDa



    10% SDS PAGE
  • Anti-PRKX antibody (ab154739) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 41 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human H3347 xenograft tissue labeling PRKX with ab154739 at 1/500 dilution.

References

ab154739 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154739.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up