Overview

  • Product nameAnti-PRKX antibody - C-terminal
    See all PRKX primary antibodies
  • Description
    Rabbit polyclonal to PRKX - C-terminal
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the C-terminal region of human PKX conjugated to KLH.

  • Positive control
    • Human lung carcinoma tissue. Jurkat cell lysate. Mouse brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab71619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • Tissue specificityHigh levels in adult and fetal brain, kidney and lung; low levels in adult placenta, heart, liver, skeletal muscle, pancreas and fetal liver.
  • Involvement in diseaseNote=A chromosomal aberration involving PRKX is a cause of sex reversal disorder. Translocation t(X;Y)(p22;p11) with PRKY. Chromosomal translocations proximal to PRKY account for about 30% of the cases of sex reversal disorder in XX males and XY females.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • PKX1 antibody
    • PRKX antibody
    • PRKX_HUMAN antibody
    • Protein kinase PKX1 antibody
    • Serine/threonine-protein kinase PRKX antibody
    see all

Anti-PRKX antibody - C-terminal images

  • All lanes : Anti-PRKX antibody - C-terminal (ab71619) at 1/100 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Mouse brain tissue lysate

    Lysates/proteins at 12.5 µg per lane.


    Predicted band size : 41 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 125 kDa,32 kDa. We are unsure as to the identity of these extra bands.
  • ab71619 at 1/10 dilution, staining PKX in human lung carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References for Anti-PRKX antibody - C-terminal (ab71619)

ab71619 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab71619.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"