Recombinant Anti-PRKX antibody [EPR4840] (ab109389)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4840] to PRKX
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PRKX antibody [EPR4840]
See all PRKX primary antibodies -
Description
Rabbit monoclonal [EPR4840] to PRKX -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: permeabilized A549 cells; WB: A549, Jurkat, 293T, fetal kidney and fetal brain lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4840 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa. |
Target
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Tissue specificity
High levels in adult and fetal brain, kidney and lung; low levels in adult placenta, heart, liver, skeletal muscle, pancreas and fetal liver. -
Involvement in disease
Note=A chromosomal aberration involving PRKX is a cause of sex reversal disorder. Translocation t(X;Y)(p22;p11) with PRKY. Chromosomal translocations proximal to PRKY account for about 30% of the cases of sex reversal disorder in XX males and XY females. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 5613 Human
- Entrez Gene: 19108 Mouse
- Entrez Gene: 501563 Rat
- Omim: 300083 Human
- SwissProt: P51817 Human
- SwissProt: Q922R0 Mouse
- Unigene: 390788 Human
- Unigene: 290338 Mouse
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Alternative names
- PKX1 antibody
- PRKX antibody
- PRKX_HUMAN antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab109389 has been referenced in 2 publications.
- Tang B et al. PEA15 promotes liver metastasis of colorectal cancer by upregulating the ERK/MAPK signaling pathway. Oncol Rep 41:43-56 (2019). PubMed: 30365128
- Ouyang C et al. Transforming Growth Factor (TGF)-ß-activated Kinase 1 (TAK1) Activation Requires Phosphorylation of Serine 412 by Protein Kinase A Catalytic Subunit a (PKACa) and X-linked Protein Kinase (PRKX). J Biol Chem 289:24226-37 (2014). WB . PubMed: 25028512