Overview

  • Product nameAnti-PRKX antibody [EPR4840]
    See all PRKX primary antibodies
  • Description
    Rabbit monoclonal [EPR4840] to PRKX
  • Tested applicationsSuitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    synthetic peptide corresponding to residues in human PRKX

  • Positive control
    • FC: permeabilized A549 cells; WB: A549, Jurkat, 293T, fetal kidney and fetal brain lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • Tissue specificityHigh levels in adult and fetal brain, kidney and lung; low levels in adult placenta, heart, liver, skeletal muscle, pancreas and fetal liver.
    • Involvement in diseaseNote=A chromosomal aberration involving PRKX is a cause of sex reversal disorder. Translocation t(X;Y)(p22;p11) with PRKY. Chromosomal translocations proximal to PRKY account for about 30% of the cases of sex reversal disorder in XX males and XY females.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • PKX1 antibody
      • PRKX antibody
      • PRKX_HUMAN antibody
      • Protein kinase PKX1 antibody
      • Serine/threonine-protein kinase PRKX antibody
      see all

    Anti-PRKX antibody [EPR4840] images

    • All lanes : Anti-PRKX antibody [EPR4840] (ab109389) at 1/1000 dilution

      Lane 1 : A549 cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : 293T cell lysates
      Lane 4 : Human fetal kidney lysates
      Lane 5 : Human fetal brain lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 41 kDa

    References for Anti-PRKX antibody [EPR4840] (ab109389)

    This product has been referenced in:
    • Ouyang C  et al. Transforming Growth Factor (TGF)-ß-activated Kinase 1 (TAK1) Activation Requires Phosphorylation of Serine 412 by Protein Kinase A Catalytic Subunit a (PKACa) and X-linked Protein Kinase (PRKX). J Biol Chem 289:24226-37 (2014). WB . Read more (PubMed: 25028512) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab109389.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"