Overview

  • Product nameAnti-PRMT1 antibody
    See all PRMT1 primary antibodies
  • Description
    Rabbit polyclonal to PRMT1
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region between residue 300 and the C terminus (residue 361) of Human PRMT1 (accession number: NP_001527.2)

  • Positive control
    • Human prostate adenocarcinoma; mouse squamous cell carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab84361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • FunctionArginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
  • Cellular localizationNucleus. Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANM 1 antibody
    • ANM1 antibody
    • ANM1_HUMAN antibody
    • HCP 1 antibody
    • HCP1 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2 antibody
    • Heterogeneous nuclear ribonucleoproteins methyltransferase like 2 antibody
    • Heterogeneous nuclear ribonucleoproteins methyltransferase like2 antibody
    • Histone-arginine N-methyltransferase PRMT1 antibody
    • HMT 2 antibody
    • HMT1 (hnRNP methyltransferase S. cerevisiae) like 2 antibody
    • HMT1 hnRNP methyltransferase antibody
    • HMT1 hnRNP methyltransferase like 2 (S. cerevisiae) antibody
    • HMT1 hnRNP methyltransferase like 2 antibody
    • HMT2 antibody
    • HRMT1 L2 antibody
    • HRMT1L 2 antibody
    • HRMT1L2 antibody
    • Human mRNA for suppressor for yeast mutant antibody
    • Human mRNA for suppressor for yeast mutant complete cds antibody
    • Interferon receptor 1 bound protein 4 antibody
    • Interferon receptor 1 bound protein4 antibody
    • Interferon receptor 1-bound protein 4 antibody
    • Interferon receptor 1bound protein 4 antibody
    • IR1 B4 antibody
    • IR1B 4 antibody
    • IR1B4 antibody
    • Mrmt 1 antibody
    • Mrmt1 antibody
    • PRMT 1 antibody
    • PRMT1 antibody
    • Protein arginine methyltransferase 1 antibody
    • Protein arginine N methyltransferase 1 antibody
    • Protein arginine N methyltransferase1 antibody
    • Protein arginine N-methyltransferase 1 antibody
    • R1B4 antibody
    • S. cerevisiae like 2 antibody
    see all

Anti-PRMT1 antibody images

  • ab84361 staining PRMT1 in mouse embryonic stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in blocking solution (PBS-T + 1% BSA)) for 1 hour at 21°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Detection of Human PRMT1 in formalin-fixed paraffin-embedded section of Human prostate adenocarcinoma by Immunohistochemistry using ab84361 at a dilution of 1/250. Detection was performed by DAB staining using anti-Rabbit IHC antibody at a dilution of 1/100.
  • Detection of Mouse PRMT1 in formalin-fixed paraffin-embedded section of Mouse squamous cell carcinoma by Immunohistochemistry using ab84361 at a dilution of 1/250. Detection was performed by DAB staining using anti-Rabbit IHC antibody at a dilution of 1/100.

References for Anti-PRMT1 antibody (ab84361)

ab84361 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Cell (embryonic stem cells)
Specification embryonic stem cells
Permeabilization Yes - 0.5% TritonX100
Fixative Paraformaldehyde
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Submitted Jun 04 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"