Anti-PRMT1 antibody [MAT-B12] - ChIP Grade (ab7027)


  • Product nameAnti-PRMT1 antibody [MAT-B12] - ChIP Grade
    See all PRMT1 primary antibodies
  • Description
    Mouse monoclonal [MAT-B12] to PRMT1 - ChIP Grade
  • SpecificityThis Mab reacts with human arginine N-Methyl Transferase
  • Tested applicationsSuitable for: Flow Cyt, WB, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein (Human)

  • Positive control
    • Tested on HELA whole cell extract and transfected Cos cells (recombinant protein PRMT1). The results showed good reactivity of the antibody in both cases.



Our Abpromise guarantee covers the use of ab7027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB 1/50 - 1/500. Predicted molecular weight: 41.48 kDa.
ChIP Use at an assay dependent concentration. (from Pubmed: 16938873)


  • FunctionArginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
  • Cellular localizationNucleus. Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANM 1 antibody
    • ANM1 antibody
    • ANM1_HUMAN antibody
    • HCP 1 antibody
    • HCP1 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2 antibody
    • Heterogeneous nuclear ribonucleoproteins methyltransferase like 2 antibody
    • Heterogeneous nuclear ribonucleoproteins methyltransferase like2 antibody
    • Histone-arginine N-methyltransferase PRMT1 antibody
    • HMT 2 antibody
    • HMT1 (hnRNP methyltransferase S. cerevisiae) like 2 antibody
    • HMT1 hnRNP methyltransferase antibody
    • HMT1 hnRNP methyltransferase like 2 (S. cerevisiae) antibody
    • HMT1 hnRNP methyltransferase like 2 antibody
    • HMT2 antibody
    • HRMT1 L2 antibody
    • HRMT1L 2 antibody
    • HRMT1L2 antibody
    • Human mRNA for suppressor for yeast mutant antibody
    • Human mRNA for suppressor for yeast mutant complete cds antibody
    • Interferon receptor 1 bound protein 4 antibody
    • Interferon receptor 1 bound protein4 antibody
    • Interferon receptor 1-bound protein 4 antibody
    • Interferon receptor 1bound protein 4 antibody
    • IR1 B4 antibody
    • IR1B 4 antibody
    • IR1B4 antibody
    • Mrmt 1 antibody
    • Mrmt1 antibody
    • PRMT 1 antibody
    • PRMT1 antibody
    • Protein arginine methyltransferase 1 antibody
    • Protein arginine N methyltransferase 1 antibody
    • Protein arginine N methyltransferase1 antibody
    • Protein arginine N-methyltransferase 1 antibody
    • R1B4 antibody
    • S. cerevisiae like 2 antibody
    see all

Anti-PRMT1 antibody [MAT-B12] - ChIP Grade images

  • Overlay histogram showing HeLa cells stained with ab7027 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7027, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-PRMT1 antibody [MAT-B12] - ChIP Grade (ab7027)

This product has been referenced in:
  • Esse R  et al. Protein arginine methylation is more prone to inhibition by S-adenosylhomocysteine than DNA methylation in vascular endothelial cells. PLoS One 8:e55483 (2013). WB ; Human . Read more (PubMed: 23408989) »
  • Slack JL  et al. Autodeimination of protein arginine deiminase 4 alters protein-protein interactions but not activity. Biochemistry 50:3997-4010 (2011). WB ; Human . Read more (PubMed: 21466234) »

See all 11 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 15 µg
Specification Hela
Blocking step Roti as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 24 2015

Thank you for your phone call. The vial that you received should be fine and you just need to add 200 ul of distilled water to reconstitute. If you have have any additional questions, please let me know.

Human PRMT1 is approximately 41kDa

We have no data yet on this antibody working in immunoprecipitation. Other antibodies that you may want to look at which are being used in chromatin immunoprecipitation (CHIP) are ab193, ab8284, and ab8721.

This antibody has never been tested in IP, therefore we can't guarantee results.

This antibody has never been tested in IP, therefore we can't guarantee results.

The molecular weight of IgG1 is approximately 150KDa, consisting of 2 heavy chains (50KDa) and 2 light chains (25KDa).

We do not have any data for its use with rat samples. However, the immunogen (PRMT1) is highly conserved across mammals so this antibody is very likely to cross react with rat.

The immunogen for this antibody was PRMT1, however we do not have data as yet on the cross-reactivity with the other PRMTs.

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