Overview

  • Product nameAnti-PRMT1 antibody [PRMT1-171]
    See all PRMT1 primary antibodies
  • Description
    Mouse monoclonal [PRMT1-171] to PRMT1
  • SpecificityThis antibody reacts with PRMT1 and does not cross-react with PRMT2, 3, 4, and 5.
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, IP, Double Immunodiffusionmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Monkey
  • Immunogen

    Monoclonal Anti-PRMT1 is derived from the PRMT1-171 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with full length human PRMT1.

  • Positive control
    • Human kidney cell line 293T.
  • General notesIf slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab12189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ELISA
IP
Double Immunodiffusion
  • Application notesDID: Use at an assay dependent dilution.
    ELISA: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 2 µg/ml (determined by using a whole cell extract of the human kidney cell line 293T). Predicted molecular weight: 42 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionArginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
    • Cellular localizationNucleus. Cytoplasm > cytosol.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANM 1 antibody
      • ANM1 antibody
      • ANM1_HUMAN antibody
      • HCP 1 antibody
      • HCP1 antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2 antibody
      • Heterogeneous nuclear ribonucleoproteins methyltransferase like 2 antibody
      • Heterogeneous nuclear ribonucleoproteins methyltransferase like2 antibody
      • Histone-arginine N-methyltransferase PRMT1 antibody
      • HMT 2 antibody
      • HMT1 (hnRNP methyltransferase S. cerevisiae) like 2 antibody
      • HMT1 hnRNP methyltransferase antibody
      • HMT1 hnRNP methyltransferase like 2 (S. cerevisiae) antibody
      • HMT1 hnRNP methyltransferase like 2 antibody
      • HMT2 antibody
      • HRMT1 L2 antibody
      • HRMT1L 2 antibody
      • HRMT1L2 antibody
      • Human mRNA for suppressor for yeast mutant antibody
      • Human mRNA for suppressor for yeast mutant complete cds antibody
      • Interferon receptor 1 bound protein 4 antibody
      • Interferon receptor 1 bound protein4 antibody
      • Interferon receptor 1-bound protein 4 antibody
      • Interferon receptor 1bound protein 4 antibody
      • IR1 B4 antibody
      • IR1B 4 antibody
      • IR1B4 antibody
      • Mrmt 1 antibody
      • Mrmt1 antibody
      • PRMT 1 antibody
      • PRMT1 antibody
      • Protein arginine methyltransferase 1 antibody
      • Protein arginine N methyltransferase 1 antibody
      • Protein arginine N methyltransferase1 antibody
      • Protein arginine N-methyltransferase 1 antibody
      • R1B4 antibody
      • S. cerevisiae like 2 antibody
      see all

    Anti-PRMT1 antibody [PRMT1-171] images

    • Immunocytochemsitry/Immunofluorescence analysis of MEFs labelling PRMT1 with ab12189. +/+ and PRMT1−/flox MEFs were treated with 2 μg/ml 4-hydroxytamoxifen (OHT) or untreated for 3 days. The cells were then seeded onto glass coverslips and cultured in fresh medium lacking OHT for 3 additional days. The cells were rinsed with ice cold methanol, fixed with 2% paraformaldehyde, and permeabilized with methanol -20°C for 5 minutes. The cells were first blocked for 20 minutes at room temperature (0.5% gelatin, 1× PBS) and then incubated with primary antibodies followed by washes (0.1% gelatin, 1% normal goat serum, 1X PBS) and incubation with Alexa Fluor 594-conjugated rabbit secondary antibody.

    References for Anti-PRMT1 antibody [PRMT1-171] (ab12189)

    This product has been referenced in:
    • Butler JS  et al. Protein-arginine Methyltransferase 1 (PRMT1) Methylates Ash2L, a Shared Component of Mammalian Histone H3K4 Methyltransferase Complexes. J Biol Chem 286:12234-44 (2011). ICC/IF ; Human . Read more (PubMed: 21285357) »
    • Infantino S  et al. Arginine methylation of the B cell antigen receptor promotes differentiation. J Exp Med 207:711-9 (2010). ICC/IF ; Mouse . Read more (PubMed: 20231378) »

    See all 3 Publications for this product

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    The monoclonal antibody ab12189 was generated using full-length human PRMT1 but to our knowledge, the epitope it recognizes has not been mapped.

    Please do not hesitate to contact us if you have other q...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"