Overview

  • Product name
  • Description
    Rabbit polyclonal to PRMT2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 8-336 of Human PRMT2 (UniProt ID: P55345).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, Molt4 and Raji whole cell lysates; A431 cells; A549 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab154154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.
  • Tissue specificity
    Widely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord.
  • Sequence similarities
    Belongs to the protein arginine N-methyltransferase family.
    Contains 1 SH3 domain.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates from the cytoplasm to the nucleus, after hormone exposure.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANM2_HUMAN antibody
    • EC 2.1.1. antibody
    • histone arginine N methyltransferase PRMT2 antibody
    • Histone-arginine N-methyltransferase PRMT2 antibody
    • HMT 1 antibody
    • HMT1 (hnRNP methyltransferase S. cerevisiae) like 1 antibody
    • HMT1 antibody
    • HMT1 hnRNP methyltransferase like 1 antibody
    • hnRNP methyltransferase (S. cerevisiae) like 1 antibody
    • hnRNP methyltransferase like 1 antibody
    • Hrmt1l1 antibody
    • MGC111373 antibody
    • PRMT 2 antibody
    • PRMT2 alpha antibody
    • prmt2 antibody
    • PRMT2 beta antibody
    • PRMT2 gamma antibody
    • PRMT2 protein antibody
    • PRMT2L2 antibody
    • Protein arginine methyltransferase 2 antibody
    • Protein arginine N methyltransferase 2 antibody
    • Protein arginine N-methyltransferase 2 antibody
    • Zf2 antibody
    see all

Images

  • Anti-PRMT2 antibody (ab154154) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 49 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PRMT2 with ab154154 at 1/200 dilution. The image in the lower panel was co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labeling PRMT2 with ab154154 at 1/100 dilution.

References

ab154154 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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