The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 37 kDa.
Approx 37kDa band observed in lysates of cell line HeLa and in rodent Brain lysates (calculated MW of 37.2kDa according to NP_001229793.1). Approx 35kDa band observed in Pig Brain lysates (calculated MW of 38.7kDa according to XP_005658963.1).
Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.
Widely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord.
Belongs to the protein arginine N-methyltransferase family. Contains 1 SH3 domain.
Cytoplasm. Nucleus. Translocates from the cytoplasm to the nucleus, after hormone exposure.