Overview

  • Product name
  • Description
    Rabbit polyclonal to PRMT3
  • Tested applications
    Suitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 432 - 481 (LEFSSDFTLK ITRTSMCTAI AGYFDIYFEK NCHNRVVFST GPQSTKTHWK) of Human PRMT3 (NP_005779)

  • Positive control
    • Fetal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ChIP Use at an assay dependent concentration.

Target

  • Function
    Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.
  • Sequence similarities
    Belongs to the protein arginine N-methyltransferase family.
    Contains 1 C2H2-type zinc finger.
  • Domain
    The zinc-finger is responsible for substrate specificity.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2010005E20Rik antibody
    • 2410018A17Rik antibody
    • AL033309 antibody
    • ANM3_HUMAN antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 3 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like 3 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 3 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3 antibody
    • HMT1 hnRNP methyltransferase like 3 antibody
    • HMT1 hnRNP methyltransferase like 3 S. cerevisiae antibody
    • HMT1-like 3 antibody
    • hnRNP methyltransferase like 3 antibody
    • HRMT1L3 antibody
    • PRMT 3 antibody
    • PRMT3 antibody
    • Protein arginine methyltransferase 3 antibody
    • Protein arginine N methyltransferase 3 antibody
    • Protein arginine N-methyltransferase 3 antibody
    see all

Images

  • Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

  • Anti-PRMT3 antibody (ab84202) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa

References

This product has been referenced in:
  • Obianyo O  et al. Activity-based protein profiling of protein arginine methyltransferase 1. ACS Chem Biol 6:1127-35 (2011). Human . Read more (PubMed: 21838253) »

See 1 Publication for this product

Customer reviews and Q&As

Filter by Application

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (u2os)
Specification
u2os
Fixative
Formaldehyde
Permeabilization
Yes - triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Dec 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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