Synthetic peptide corresponding to a region within internal sequence amino acids 432 - 481 (LEFSSDFTLK ITRTSMCTAI AGYFDIYFEK NCHNRVVFST GPQSTKTHWK) of Human PRMT3 (NP_005779)
Our Abpromise guarantee covers the use of ab84202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ChIP||Use at an assay dependent concentration.|
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
ab84202 has not yet been referenced specifically in any publications.