Anti-PRMT3 antibody - ChIP Grade (ab84202)

Overview

  • Product nameAnti-PRMT3 antibody - ChIP Grade
    See all PRMT3 primary antibodies
  • Description
    Rabbit polyclonal to PRMT3 - ChIP Grade
  • Tested applicationsSuitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 432 - 481 (LEFSSDFTLK ITRTSMCTAI AGYFDIYFEK NCHNRVVFST GPQSTKTHWK) of Human PRMT3 (NP_005779)

  • Positive control
    • Fetal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ChIP Use at an assay dependent concentration.

Target

  • FunctionMethylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.
  • Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
    Contains 1 C2H2-type zinc finger.
  • DomainThe zinc-finger is responsible for substrate specificity.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2010005E20Rik antibody
    • 2410018A17Rik antibody
    • AL033309 antibody
    • ANM3_HUMAN antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 3 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like 3 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 3 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3 antibody
    • HMT1 hnRNP methyltransferase like 3 antibody
    • HMT1 hnRNP methyltransferase like 3 S. cerevisiae antibody
    • HMT1-like 3 antibody
    • hnRNP methyltransferase like 3 antibody
    • HRMT1L3 antibody
    • PRMT 3 antibody
    • PRMT3 antibody
    • Protein arginine methyltransferase 3 antibody
    • Protein arginine N methyltransferase 3 antibody
    • Protein arginine N-methyltransferase 3 antibody
    see all

Anti-PRMT3 antibody - ChIP Grade images

  • Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

  • Anti-PRMT3 antibody - ChIP Grade (ab84202) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa

References for Anti-PRMT3 antibody - ChIP Grade (ab84202)

ab84202 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cultured Cells (u2os)
Specification u2os
Fixative Formaldehyde
Permeabilization Yes - triton
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"