• Product name
  • Description
    Rabbit polyclonal to PRMT4
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus laevis
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 48-63 of Human PRMT4 conjugated to KLH via a C terminal added lysine residue.

  • Positive control
    • WB: nuclear extracts of MCF7 breast carcinoma cell line. IP: extracts of transfected 293T cells.


Our Abpromise guarantee covers the use of ab50214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IP: Use at 5-20 µg.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 63 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
    • Tissue specificity
      Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
    • Sequence similarities
      Belongs to the protein arginine N-methyltransferase family.
    • Post-translational
      Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing.
      Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol.
    • Cellular localization
      Nucleus. Cytoplasm. Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • carm1 antibody
      • CARM1_HUMAN antibody
      • Coactivator associated arginine methyltransferase 1 antibody
      • Coactivator-associated arginine methyltransferase 1 antibody
      • Histone arginine methyltransferase CARM 1 antibody
      • Histone arginine methyltransferase CARM1 antibody
      • Histone-arginine methyltransferase CARM1 antibody
      • PRMT 4 antibody
      • PRMT4 antibody
      • Protein arginine methyltransferase antibody
      • Protein arginine N methyltransferase 4 antibody
      • Protein arginine N-methyltransferase 4 antibody
      see all

    Anti-PRMT4 antibody images

    • All lanes : Anti-PRMT4 antibody (ab50214) at 1 µg/ml

      Lane 1 : Nuclear extracts of MCF7 breast carcinoma cells
      Lane 2 : Nuclear extracts of MCF7 breast carcinoma cells with PRMT4 immunizing peptide at 10 µg/ml

      Goat anti-Rabbit IgG, Peroxidase conjugate
      Developed using the ECL technique

      Predicted band size : 63 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    References for Anti-PRMT4 antibody (ab50214)

    This product has been referenced in:
    • Benedikt A  et al. The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures. Leukemia 25:135-44 (2011). IP ; Human . Read more (PubMed: 21030982) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Human Cell lysate - whole cell (Prostata Cancer Cells)
    Loading amount
    10 µg
    Prostata Cancer Cells
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

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    Verified customer

    Submitted Oct 07 2009


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