Overview

  • Product nameAnti-PRMT4 antibodySee all PRMT4 primary antibodies ...
  • Description
    Rabbit polyclonal to PRMT4
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 550-585 of Human PRMT4 (SwissProt Q86X55).

  • Positive control
    • Human breast adenocarcinoma. Human prostate adenocarcinoma.

Applications

Our Abpromise guarantee covers the use of ab84370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notesIHC-P: 1/100 - 1/500.

    Antigen exposure is recommended.
    Antigen exposure with citrate buffer will enhance staining.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMethylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
    • Tissue specificityOverexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
    • Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
    • Post-translational
      modifications
      Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing.
      Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol.
    • Cellular localizationNucleus. Cytoplasm. Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CARM1 antibody
      • CARM1_HUMAN antibody
      • Coactivator associated arginine methyltransferase 1 antibody
      • Coactivator-associated arginine methyltransferase 1 antibody
      • Histone arginine methyltransferase CARM 1 antibody
      • Histone arginine methyltransferase CARM1 antibody
      • Histone-arginine methyltransferase CARM1 antibody
      • PRMT 4 antibody
      • PRMT4 antibody
      • Protein arginine methyltransferase antibody
      • Protein arginine N methyltransferase 4 antibody
      • Protein arginine N-methyltransferase 4 antibody
      see all

    Anti-PRMT4 antibody images

    • ab84370 at 1/100, staining human PRMT4 in a section of human breast adenocarcinoma by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    • ab84370 at 1/500, staining human PRMT4 in a section of human prostate adenocarcinoma by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    References for Anti-PRMT4 antibody (ab84370)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (u2os)
    Loading amount 80 µg
    Specification u2os
    Gel Running Conditions Reduced Denaturing (4-20)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 10 2011

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cultured Cells (u2os)
    Specification u2os
    Fixative Formaldehyde
    Permeabilization Yes - triton
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 14 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"