• Product nameAnti-PRMT5 antibody
    See all PRMT5 primary antibodies
  • Description
    Rabbit polyclonal to PRMT5
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig
  • Immunogen

    A region within synthetic peptide: GFDFLCMPVF HPRFKREFIQ EPAKNRPGPQ TRSDLLLSGR DWNTLIVGKL, corresponding to N terminal amino acids 37-86 of Human PRMT5

  • Positive control
    • Jurkat cell lysate, human kidney tissue, human lung tissue.



Our Abpromise guarantee covers the use of ab50933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 72 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/62500.

Titre using peptide based assay: 1/62500.

IHC-P Use a concentration of 4 - 8 µg/ml.


  • FunctionArginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
  • Post-translational
    Disulfide bonds and non-covalent association mediate homooligomers formation.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 72 kDa ICln binding protein antibody
    • 72 kDa ICln-binding protein antibody
    • ANM5_HUMAN antibody
    • Histone synthetic lethal 7, S. cerevisiae, homolog of antibody
    • Histone-arginine N-methyltransferase PRMT5 antibody
    • HMT1 hnRNP methyltransferase like 5 antibody
    • HOMOLOG OF; SKB1 antibody
    • HRMT1L5 antibody
    • IBP72 antibody
    • Jak-binding protein 1 antibody
    • JBP 1 antibody
    • JBP1 antibody
    • PRMT 5 antibody
    • PRMT5 antibody
    • Protein arginine methyltransferase 5 antibody
    • Protein arginine N methyltransferase 5 antibody
    • Protein arginine N methyltransferase 5 N terminally processed antibody
    • Protein arginine N-methyltransferase 5 antibody
    • S. POMBE antibody
    • S. POMBE HOMOLOG OF; SKB1 antibody
    • Shk1 kinase binding protein 1 homolog antibody
    • Shk1 kinase-binding protein 1 homolog antibody
    • Shk1 kinase/binding protein 1, S. pombe, homolog of antibody
    • SKB 1 antibody
    • SKB1 antibody
    • SKB1 homolog antibody
    • SKB1: SKB1 homolog (S. pombe) antibody
    • SKB1Hs antibody
    see all

Anti-PRMT5 antibody images

  • Anti-PRMT5 antibody (ab50933) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 72 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Ab50933 at 8µg/ml staining epithelial cells of renal tubule from paraffin embedded human kidney.
  • Ab50933 at 8µg/ml staining alveolar cells from paraffin embedded human lung.

References for Anti-PRMT5 antibody (ab50933)

ab50933 has not yet been referenced specifically in any publications.

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