Overview

  • Product nameAnti-PRMT5 antibody
    See all PRMT5 primary antibodies
  • Description
    Rabbit polyclonal to PRMT5
  • Tested applicationsSuitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 50 and 100 of human PRMT5 (NP_006100.2)

  • Positive control
    • HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionArginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
    • Post-translational
      modifications
      Disulfide bonds and non-covalent association mediate homooligomers formation.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 72 kDa ICln binding protein antibody
      • 72 kDa ICln-binding protein antibody
      • ANM5_HUMAN antibody
      • Histone synthetic lethal 7, S. cerevisiae, homolog of antibody
      • Histone-arginine N-methyltransferase PRMT5 antibody
      • HMT1 hnRNP methyltransferase like 5 antibody
      • HOMOLOG OF; SKB1 antibody
      • HRMT1L5 antibody
      • IBP72 antibody
      • Jak-binding protein 1 antibody
      • JBP 1 antibody
      • JBP1 antibody
      • PRMT 5 antibody
      • PRMT5 antibody
      • Protein arginine methyltransferase 5 antibody
      • Protein arginine N methyltransferase 5 antibody
      • Protein arginine N methyltransferase 5 N terminally processed antibody
      • Protein arginine N-methyltransferase 5 antibody
      • S. POMBE antibody
      • S. POMBE HOMOLOG OF; SKB1 antibody
      • SHK1 KINASE BINDING PROTEIN 1 antibody
      • Shk1 kinase binding protein 1 homolog antibody
      • Shk1 kinase-binding protein 1 homolog antibody
      • Shk1 kinase/binding protein 1, S. pombe, homolog of antibody
      • SKB 1 antibody
      • SKB1 antibody
      • SKB1 homolog antibody
      • SKB1: SKB1 homolog (S. pombe) antibody
      • SKB1Hs antibody
      see all

    Anti-PRMT5 antibody images

    • IHC image of ab70644 staining in human colonic carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70644, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References for Anti-PRMT5 antibody (ab70644)

    ab70644 has not yet been referenced specifically in any publications.

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