Recombinant
RabMAb

Anti-PRMT6 antibody [EPR18500] (ab190902)

Overview

  • Product name
    Anti-PRMT6 antibody [EPR18500]
    See all PRMT6 primary antibodies
  • Description
    Rabbit monoclonal [EPR18500] to PRMT6
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PRMT6 aa 200-300. The exact sequence is proprietary.
    Database link: Q96LA8

  • Positive control
    • PRMT6 with GFP tag transfected HEK-293T whole cell lysate; HeLa, LLC, Jurkat, HEK-293, K562, MCF7, LNCaP, C2C12, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; human fetal heart, fetal kidney and fetal spleen lysates; rat brain, heart, kidney and spleen lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab190902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

  • Function
    Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53. Repression of TP53 blocks cellular senescence (By similarity). Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC).
  • Tissue specificity
    Highly expressed in kidney and testis.
  • Sequence similarities
    Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT6 subfamily.
    Contains 1 SAM-dependent MTase PRMT-type domain.
  • Post-translational
    modifications
    Automethylation enhances its stability and antiretroviral activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANM6_HUMAN antibody
    • Chromobox protein homolog 7 antibody
    • FLJ10559 antibody
    • FLJ51477 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6 antibody
    • Histone-arginine N-methyltransferase PRMT6 antibody
    • HMT1 hnRNP methyltransferase like 6. antibody
    • HRMT1L6 antibody
    • OTTHUMP00000012633 antibody
    • PRMT 6 antibody
    • prmt6 antibody
    • Protein arginine methyltransferase 6 antibody
    • Protein arginine N methyltransferase 6 antibody
    • Protein arginine N-methyltransferase 6 antibody
    see all

Images

  • All lanes : Anti-PRMT6 antibody [EPR18500] (ab190902) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T(Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : Empty vector with GFP tag (vector control) transfected HEK-293T(Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : PRMT6 with GFP tag transfected HEK-293T(Human epithelial cell line from embryonic kidney) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed 75 kDa band in lane 1 and 2 is an extra band, and the observed 68 kDa in lane 3 is the fusion protein (PRMT6 + GFP). The 48 kDa band in lane 3 is probably degraded fusion protein (PRMT6 + GFP). Endogenous PRMT6 (42 kDa) can be observed in all three lanes.

  • All lanes : Anti-PRMT6 antibody [EPR18500] (ab190902) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : LLC (Mouse lung carcinoma cell line) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 5 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 7 : LNCaP (Human prostate cancer cell line) whole cell lysate
    Lane 8 : C2C12 (Mouse myoblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 3 minutes

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-PRMT6 antibody [EPR18500] (ab190902) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 3 minutes

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-PRMT6 antibody [EPR18500] (ab190902) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 8 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 3 minutes

    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab190902 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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