The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/100 - 1/500.
FunctionArginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo.
Involvement in diseaseDefects in PRMT7 are associated with mild intellectual disabilityy, obesity and symmetrical shortening of the digits and posterior metacarpals and metatarsals. The phenotype is a phenocopy of pseudohypoparathyroidism (PHP).
Sequence similaritiesBelongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT7 subfamily. Contains 2 SAM-dependent MTase PRMT-type domains.
ab59891, at 1/50 dilution, staining PRMT7 in formalin-fixed, paraffin-embedded human hepatocarcinoma tissue by Immunohistochemistry. The primary antibody was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Western blot - PRMT7 antibody (ab59891)
All lanes : Anti-PRMT7 antibody (ab59891) at 1/500 dilution
Lane 1 : 293 cell lysate non-transfected Lane 2 : 293 cell lysate transiently transfected with the PRMT7 gene
Lysates/proteins at 2 µg per lane.
References for Anti-PRMT7 antibody (ab59891)
has not yet been referenced specifically in any publications.
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