Anti-PRMT8 antibody - Carboxyterminal end (ab70899)

Overview

  • Product nameAnti-PRMT8 antibody - Carboxyterminal end
    See all PRMT8 primary antibodies
  • Description
    Rabbit polyclonal to PRMT8 - Carboxyterminal end
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PRMT8 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • K562 cell lysate, 293 cell lysates transiently transfected with the PRMT8 gene

Properties

Applications

Our Abpromise guarantee covers the use of ab70899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ELISA 1/1000.

Target

  • FunctionMembrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS.
  • Tissue specificityBrain-specific.
  • Sequence similaritiesBelongs to the protein arginine N-methyltransferase family. PRMT8 subfamily.
  • DomainThe SH3-binding motifs mediate the interaction with SH3 domain-containing proteins such as PRMT2 and FYN, possibly leading to displace the N-terminal domain and activate the protein.
    The N-terminal region (1-60) inhibits the enzymatic activity.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANM8_HUMAN antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 4 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4 antibody
    • HMT1 hnRNP methyltransferase like 3 antibody
    • HMT1 hnRNP methyltransferase like 4 antibody
    • HRMT1 L3 antibody
    • HRMT1 L4 antibody
    • HRMT1L 3 antibody
    • HRMT1L 4 antibody
    • HRMT1L3 antibody
    • HRMT1L4 antibody
    • prmt8 antibody
    • Protein arginine N methyltransferase 4 antibody
    • Protein arginine N methyltransferase 8 antibody
    • Protein arginine N-methyltransferase 8 antibody
    see all

Anti-PRMT8 antibody - Carboxyterminal end images

  • Anti-PRMT8 antibody - Carboxyterminal end (ab70899) at 1/60 dilution + K562 cell lysate at 35 µg

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 100 kDa,55 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-PRMT8 antibody - Carboxyterminal end (ab70899) at 1/50 dilution

    Lane 1 : Non transfected 293 cell lysate
    Lane 2 : PRMT8 transfected 293 cell lysate

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 120 kDa. We are unsure as to the identity of these extra bands.

References for Anti-PRMT8 antibody - Carboxyterminal end (ab70899)

ab70899 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Astatotilapia burtoni Tissue sections (Preoptic area of the brain)
Antigen retrieval step None
Permeabilization Yes - 0.2% tritonX
Specification Preoptic area of the brain
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
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Submitted Sep 30 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"