The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
FunctionMembrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS.
Sequence similaritiesBelongs to the protein arginine N-methyltransferase family. PRMT8 subfamily.
DomainThe SH3-binding motifs mediate the interaction with SH3 domain-containing proteins such as PRMT2 and FYN, possibly leading to displace the N-terminal domain and activate the protein. The N-terminal region (1-60) inhibits the enzymatic activity.