Overview

  • Product name
    Anti-PRNT antibody
  • Description
    Rabbit polyclonal to PRNT
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: LDASIHPFRL PFSSKPFLLI PMSNTTLPHT AWPLSFLHQT VSTLKAVAVT HSLWHLQIPV DCQACNR, corresponding to internal sequence amino acids 21-87 of Human PRNT (Q86SH4).

  • Positive control
    • Human testis tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • M8 antibody
    • Prion protein (testis specific) antibody
    • Protein M8 antibody
    • Putative testis-specific prion protein antibody

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli and cytoskeleton (cytokinetic bridge). Recommended concentration of ab126413 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab126413, at a 1/20 dilution, staining PRNT in paraffin embedded Human testis tissue by Immunohistochemistry.

References

ab126413 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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