![Western blot - pro Caspase 10 antibody [E35] (ab32155) image](#ab32155wb.gif)
![Immunohistochemistry (Paraffin-embedded sections) - pro Caspase 10 antibody [E35] (ab32155) image](#ab32155ihc.jpg)
![Immunocytochemistry/ Immunofluorescence - pro Caspase 10 antibody [E35] (ab32155) image](#ab32155if.jpg)
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- Secondary antibodies
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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-pro Caspase 10 antibody [E35]
Description
Rabbit monoclonal [E35] to pro Caspase 10
Specificity
The antibody does not crossreact with other Caspase family members.
Tested applications
ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Does not react with
Rat
Immunogen
A synthetic peptide corresponding to N terminal residues of human Caspase 10 subunit p23/17.
Positive control
WB: Hela cell lysate. IF and FACS: Hela cells. IHC-P: Human breast carcinoma tissue.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
E35
Isotype
IgG
Research areas
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/100.
WB: 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
IHC-P: 1/50 - 1/100.
Flow Cyt: 1/50.
IP
IP: 1/10.IP: 1/10.
Target
Relevance
Caspases are a family of cytosolic aspartate specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase 10 (MCH4, FLICE2) is one of the initiator caspases, and is localized to various tissues. Pro Caspase 10 has two death effector domains (DEDs) that bind FADD and recruits both TNFR1 and CD95 to form complexes with these receptors and induce apoptosis. This death domain complex cleaves pro Caspase 10 into a large active fragment and a small fragment. Cleaved Caspase 10 leads to the processing of Caspase 3 and Caspase 7, initiating a caspase cascade and subsequent apoptosis.
Alternative names
- Apoptosis related cysteine peptidase antibody
- Apoptotic protease Mch 4 antibody
- Apoptotic protease Mch4 antibody
see all
Database links
- Entrez Gene: 843 Human
- Omim: 601762 Human
- SwissProt: Q92851 Human
- SwissProt: Q8IUP5 Human
- Unigene: 5353 Human
Anti-pro Caspase 10 antibody [E35] images:
Western blot - pro Caspase 10 antibody [E35] (ab32155)
![Western blot - pro Caspase 10 antibody [E35] (ab32155)](/ps/datasheet/Images/32/ab32155/ab32155wb.gif)
Anti-pro Caspase 10 antibody [E35] (ab32155) at 1/1000 dilution + Hela cell lysate
Predicted band size : 59 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - pro Caspase 10 antibody [E35] (ab32155)
![Immunohistochemistry (Paraffin-embedded sections) - pro Caspase 10 antibody [E35] (ab32155)](/ps/datasheet/Images/32/ab32155/ab32155ihc.jpg)
Ab32155, at 1/50 dilution, staining pro Caspase 10 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - pro Caspase 10 antibody [E35] (ab32155)
![Immunocytochemistry/ Immunofluorescence - pro Caspase 10 antibody [E35] (ab32155)](/ps/datasheet/Images/32/ab32155/ab32155if.jpg)
Ab32155, at 1/100 dilution, staining pro Caspase 10 in apoptotic HeLa cells by Immunofluorescence.
Flow Cytometry / FACS - pro Caspase 10 antibody [E35] (ab32155)
![Flow Cytometry / FACS - pro Caspase 10 antibody [E35] (ab32155)](/ps/datasheet/Images/32/ab32155/ab32155facs.gif)
Flow cytometric analysis of permeabilized HeLa cells using ab32155 at a 1/50 dilution (red) or a rabbit IgG (negative)(green).
References for Anti-pro Caspase 10 antibody [E35] (ab32155)
ab32155 has not yet been referenced specifically in any publications.
Publishing research using ab32155? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"