Anti-pro Caspase 3 antibody [E61] (ab32150)

  Western blot - pro Caspase 3 antibody [E61] (ab32150)

Anti-pro Caspase 3 antibody [E61] (ab32150) at 1/1000 dilution + Hela cell lysate

Observed band size : 35 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - pro Caspase 3 antibody [E61] (ab32150)

Immunohistochemical analysis of human paraffin-embedded cervical carcinoma tissue using ab32150 at 1/500 dilution.

  Immunocytochemistry/ Immunofluorescence - pro Caspase 3 antibody [E61] (ab32150)

Immunofluorescent staining of HeLa cells using ab32150 at 1/100 dilution.

  Flow Cytometry-Anti-pro Caspase 3 antibody [E61](ab32150)

Overlay histogram showing Jurkat cells stained with ab32150 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32150, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (1µg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.