![Western blot - pro Caspase 3 antibody [Y83-103] (ab32499) image](#ab32499wb.gif)
![Immunohistochemistry (Paraffin-embedded sections) - pro Caspase 3 antibody [Y83-103] (ab32499) image](#ab32499ihc.jpg)
 image](#pro-Caspase-3-Primary-antibodies-ab32499-1.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
- Lysates
- Slides
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-pro Caspase 3 antibody [E83-103]
See all pro Caspase 3 products (3) ...
Description
Rabbit monoclonal [E83-103] to pro Caspase 3
Specificity
This antibody only detects pro-form (35kD) of caspase 3, and does not recognize any cleaved caspases.
Tested applications
ICC/IF, WB, IHC-P, IP, Flow Cyt, ICCmore details
Cross reactivity
Reacts with
Mouse, Human
Does not react with
Rat
Immunogen
A synthetic peptide corresponding to residues following Ser29 of human caspase-3 (N-terminus of p17 subunit).
Positive control
Jurkat cell lysate and human colon adenocarcinoma.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
E83-103
Isotype
IgG
Research areas
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml.
WB: 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).
IHC-P: 1/250.
IP
IP: 1/100.IP: 1/100.
Flow Cyt
Flow Cyt: 1/50.Flow Cyt: 1/50.
ICC
ICC: 1/250.ICC: 1/250.
Target
Relevance
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase 3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase 3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase 3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase 3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.
Cellular localization
Cytoplasmic
Alternative names
- APOPAIN antibody
- Apoptosis related cysteine peptidase antibody
- CASP 3 antibody
see all
Database links
- Entrez Gene: 836 Human
- Entrez Gene: 12367 Mouse
- Omim: 600636 Human
- SwissProt: P42574 Human
- SwissProt: P70677 Mouse
- Unigene: 141125 Human
- Unigene: 34405 Mouse
Anti-pro Caspase 3 antibody [E83-103] images:
Western blot - pro Caspase 3 antibody [Y83-103] (ab32499)
![Western blot - pro Caspase 3 antibody [Y83-103] (ab32499)](/ps/datasheet/Images/32/ab32499/ab32499wb.gif)
All lanes : Anti-pro Caspase 3 antibody [E83-103] (ab32499) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate + Camptothecin
Predicted band size : 31 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - pro Caspase 3 antibody [Y83-103] (ab32499)
![Immunohistochemistry (Paraffin-embedded sections) - pro Caspase 3 antibody [Y83-103] (ab32499)](/ps/datasheet/Images/32/ab32499/ab32499ihc.jpg)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma ab32499 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence-pro Caspase 3 antibody [E83-103](ab32499)
](/ps/datasheet/images/32/ab32499/pro-Caspase-3-Primary-antibodies-ab32499-1.jpg)
ICC/IF image of ab32499 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32499, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-pro Caspase 3 antibody [E83-103] (ab32499)
ab32499 has not yet been referenced specifically in any publications.
Publishing research using ab32499? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"