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Anti-pro Caspase 9 antibody [E84] (ab32068)

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Overview

Product name

Anti-pro Caspase 9 antibody [E84]
See all pro Caspase 9 products (2) ...

Description

Rabbit monoclonal [E84] to pro Caspase 9

Specificity

This antibody only recognises the pro form of Caspase 9 (46kDa)

Tested applications

WB, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresponding to residues near N-terminus of human Caspase-9

Positive control

Jurkat cell lysate

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

E84

Isotype

IgG

Research areas

Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases

  • Western blot - pro Caspase 9 antibody [E84] (ab32068)Western blot - pro Caspase 9 antibody [E84] (ab32068) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/2000. Detects a ba...Read more →

    WB: 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

  • Flow Cyt

     Flow Cyt: 1/50.

    Flow Cyt: 1/50.

Application notes

Is unsuitable for ,ICC or IP.

Target

Relevance

Pro Caspase 9 exists as a 46 kDa isomer that is cleaved by apoptotic stimulation, and functions as the major initiator caspase in programmed cell death, or apoptosis. Upon stimulation, cytochrome c is released from mitochondria, and associates with the Pro Caspase 9/APAF1 complex. This complex in turn activates Pro Caspase 9 by cleaving it into an active 35 kDa fragment and a small 10 kDa fragment. Activated Caspase 9 cleaves Caspase 3 as well as poly (ADP-ribose) polymerase (PARP) to initiate a caspase cascade and apoptosis.

Alternative names

  • APAF3 antibody
  • Apoptotic Protease Activating Factor 3 antibody
  • Apoptotic Protease Mch 6 antibody
  • CASP9 antibody
  • Caspase 9 Precursor antibody
  • Caspase 9 Subunit p10 antibody
  • Caspase 9 Subunit p35 antibody
  • ICE Like Apoptotic Protease 6 antibody
  • ICELAP6 antibody
  • MCH6 antibody
see all

Database links

Anti-pro Caspase 9 antibody [E84] images:

  Western blot - pro Caspase 9 antibody [E84] (ab32068)

Western blot - pro Caspase 9 antibody [E84] (ab32068)

Anti-pro Caspase 9 antibody [E84] (ab32068) at 1/2000 dilution + Jurkat cell lysate at 10 µg

Predicted band size : 46 kDa
Observed band size : 46 kDa

References for Anti-pro Caspase 9 antibody [E84] (ab32068)

This product has been referenced in:

See 1 publication for this product

Publishing research using ab32068? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"