• Product nameAnti-pro Caspase 10 antibody [E35]
  • Description
    Rabbit monoclonal [E35] to pro Caspase 10
  • SpecificityThe antibody does not crossreact with other Caspase family members.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to N terminal residues of human Caspase 10 subunit p23/17.

  • Positive control
    • WB: Hela cell lysate. IF and FACS: Hela cells. IHC-P: Human breast carcinoma tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • ClonalityMonoclonal
  • Clone numberE35
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab32155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/10.


  • RelevanceCaspases are a family of cytosolic aspartate specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase 10 (MCH4, FLICE2) is one of the initiator caspases, and is localized to various tissues. Pro Caspase 10 has two death effector domains (DEDs) that bind FADD and recruits both TNFR1 and CD95 to form complexes with these receptors and induce apoptosis. This death domain complex cleaves pro Caspase 10 into a large active fragment and a small fragment. Cleaved Caspase 10 leads to the processing of Caspase 3 and Caspase 7, initiating a caspase cascade and subsequent apoptosis.
  • Database links
  • Alternative names
    • Apoptotic protease Mch4 antibody
    • CASP10 antibody
    • Caspase 10 antibody
    • FLICE2 antibody
    • ICE like apoptotic protease 4 antibody
    see all

Anti-pro Caspase 10 antibody [E35] images

  • Anti-pro Caspase 10 antibody [E35] (ab32155) at 1/1000 dilution + Hela cell lysate

    Predicted band size : 59 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Ab32155, at 1/50 dilution, staining pro Caspase 10 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
  • Ab32155, at 1/100 dilution, staining pro Caspase 10 in apoptotic HeLa cells by Immunofluorescence.
  • Flow cytometric analysis of permeabilized HeLa cells using ab32155 at a 1/50 dilution (red) or a rabbit IgG (negative)(green).

References for Anti-pro Caspase 10 antibody [E35] (ab32155)

This product has been referenced in:

See 1 Publication for this product

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