Anti-pro Caspase-12 antibody (ab8117)

Overview

  • Product nameAnti-pro Caspase-12 antibody
    See all pro Caspase-12 primary antibodies
  • Description
    Rabbit polyclonal to pro Caspase-12
  • SpecificityThe antibody only recognizes the prodomain, not the activated form.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse pro Caspase-12 aa 100-116.
    Sequence:

    CTPSSPSESKRKVEDDE


    Database link: Q6UXS9
    (Peptide available as ab8374)

  • Positive control
    • Mouse brain tissue lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage bufferPBS with 0.02% sodium azide
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab8117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa.Can be blocked with Mouse Caspase-12 peptide (ab8374).

Target

  • RelevanceCaspase 12 is activated by ER stress, including disruption of ER calcium homeostasis and mediates ER stress-induced apoptosis. Caspase 12 is co-localized to the ER with several proteins that are involved in Alzheimers disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase 12 mediates cytotoxicity induced by amyloid-beta. It is ubiquitously expressed in mouse tissue.
  • Cellular localizationEndoplasmic reticulum
  • Database links
  • Alternative names
    • CASP12 antibody
    • CASP12P1 antibody

Anti-pro Caspase-12 antibody images

  • All lanes : Anti-pro Caspase-12 antibody (ab8117) at 1 µg/ml

    Lane 1 : Murine brain tissue lysate with absence of blocking peptide
    Lane 2 : Murine brain tissue lysate with presence of blocking peptide
  • ab8117 staining Caspase-12 in mouse liver tissue by IHC

  • Immunofluorescence of Caspase-12 in Mouse Liver cells using ab8117 at 10 ug/ml.

References for Anti-pro Caspase-12 antibody (ab8117)

ab8117 has not yet been referenced specifically in any publications.

Product Wall

We do not have an Italian distributor but you can place your orders directly through us. This can be done online, by fax (0033 1223 472038), phone (0033 1223 472030) or email (orders@abcam.com). Ab8117 does cross-react with human. Unfortunately I ...

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Thank you very much for your enquiry. For both ab8117 and ab10455, the band should be at about 50-55 kDa. If you have any further questions, please contact us again.

This antibody recognizes the uncleaved (whole) form of the caspase only.

This antibody recognizes the uncleaved (whole) form of the caspase only.

No, I'm afraid that we do not have an blot image for this antibody. All the information we have is on the datasheet.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"