Overview

  • Product name
    Anti-pro Caspase-12 antibody
    See all pro Caspase-12 primary antibodies
  • Description
    Rabbit polyclonal to pro Caspase-12
  • Specificity
    The antibody only recognizes the prodomain, not the activated form.
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Does not react with: Human
  • Immunogen

    Synthetic peptide corresponding to Mouse pro Caspase-12 aa 2-17 (N terminal).
    Sequence:

    AARRTHERDPIYKIKG


    Database link: Q6UXS9
    (Peptide available as ab8375)

  • Positive control
    • Mouse spleen tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    PBS with 0.02% sodium azide
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa.Can be blocked with Mouse Caspase-12 peptide (ab8375).

Target

  • Relevance
    Caspase 12 is activated by ER stress, including disruption of ER calcium homeostasis and mediates ER stress-induced apoptosis. Caspase 12 is co-localized to the ER with several proteins that are involved in Alzheimers disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase 12 mediates cytotoxicity induced by amyloid-beta. It is ubiquitously expressed in mouse tissue.
  • Cellular localization
    Endoplasmic reticulum
  • Database links
  • Alternative names
    • CASP12 antibody
    • CASP12P1 antibody

Images

  • All lanes : Anti-pro Caspase-12 antibody (ab8118) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate
    Lane 2 : Mouse spleen tissue lysate
  • ab8118 at 10µg/ml staining Caspase-12 in human heart tissue by IHC

References

This product has been referenced in:
  • Hauri-Hohl MM  et al. Donor T-cell alloreactivity against host thymic epithelium limits T-cell development after bone marrow transplantation. Blood 109:4080-8 (2007). Read more (PubMed: 17213290) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your e-mail. Did you mean ab24852, rather than 24582? The antibodies are against caspase 12, not caspase 1. Can you please confirm that the customer is looking for caspase 12 and not caspase 1? I compared the two sheets of ab24852 and...

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I'm sorry to hear your customer is experiencing problems with ab8118. The customer mentions that he/she provided images however these did not reach us, so I cannot see the membrane pattern with this antibody I'm afraid. It is very unclear what ...

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This antibody is raised against the N-terminal sequence AARRTHERDPIYKIKG. If this sequence forms part of the pro-peptide, then this antibody will be selective for the non-activated form. The blocking peptide for this antibody is available separatel...

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These two antibodies ab8118 and ab8117 recognize the pre-activated form of caspase 12, however we have not tested for the activated/cleaved form.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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