Anti-pro Caspase 7 antibody [Y33] (ab32067)

Overview

  • Product nameAnti-pro Caspase 7 antibody [Y33]
  • Description
    Rabbit monoclonal [Y33] to pro Caspase 7
  • SpecificityThe antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to N-terminal residues of human Pro-Caspase-7.

  • Positive control
    • Jurkat cell lysate and human lung squamous carcinoma.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab32067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notesICC/IF: 1/50.
    IHC-P: 1/50.

    WB: 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

    Is unsuitable for FACS or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
      -Gly-217' bond. Overexpression promotes programmed cell death.
    • Tissue specificityHighly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
    • Sequence similaritiesBelongs to the peptidase C14A family.
    • Post-translational
      modifications
      Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • apoptosis-related cysteine peptidase antibody
      • Apoptotic protease Mch-3 antibody
      • CASP-7 antibody
      • CASP7 antibody
      • CASP7_HUMAN antibody
      • Caspase-7 subunit p11 antibody
      • CMH-1 antibody
      • ICE-LAP3 antibody
      • ICE-like apoptotic protease 3 antibody
      see all

    Anti-pro Caspase 7 antibody [Y33] images

    • Anti-pro Caspase 7 antibody [Y33] (ab32067) at 1/1000 dilution + Jurkat cell lysate

      Predicted band size : 34 kDa
      Observed band size : 34 kDa
    • Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma using ab32067 at 1/50 dilution.

    References for Anti-pro Caspase 7 antibody [Y33] (ab32067)

    ab32067 has not yet been referenced specifically in any publications.

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