Validated using a knockout cell line
Recombinant
RabMAb

Anti-pro Caspase 7 antibody [Y33] (ab32067)

Overview

  • Product name
    Anti-pro Caspase 7 antibody [Y33]
  • Description
    Rabbit monoclonal [Y33] to pro Caspase 7
  • Specificity
    The antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to N-terminal residues of human Pro-Caspase-7.

  • Positive control
    • Jurkat cell lysate and human lung squamous carcinoma.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notes
    ICC/IF: 1/50.
    IHC-P: 1/50.

    WB: 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

    Is unsuitable for FACS or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
      -Gly-217' bond. Overexpression promotes programmed cell death.
    • Tissue specificity
      Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
    • Sequence similarities
      Belongs to the peptidase C14A family.
    • Post-translational
      modifications
      Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • apoptosis-related cysteine peptidase antibody
      • Apoptotic protease Mch-3 antibody
      • CASP-7 antibody
      • CASP7 antibody
      • CASP7_HUMAN antibody
      • Caspase-7 subunit p11 antibody
      • CMH-1 antibody
      • ICE-LAP3 antibody
      • ICE-like apoptotic protease 3 antibody
      see all

    Anti-pro Caspase 7 antibody [Y33] images



    • Predicted band size : 34 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Staurosporin treated HAP1 whole cell lysate (20 µg)
      Lane 3: CASP7 knockout HAP1 whole cell lysate (20 µg)
      Lane 4: Staurosporin treated CASP7 knockout HAP1 whole cell lysate (20 µg)
      Lane 5: HeLa whole cell lysate (20 µg)
      Lane 6: Staurosporin treated HeLa whole cell lysate (20 µg)

      Lanes 1 - 6: Merged signal (red and green). Green - ab32067 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab32067 was shown to recognize pro Caspase 7 when HAP1 + pro Caspase 7 knockout samples were used, along with additional cross-reactive bands. Wild-type and pro Caspase 7 knockout samples were subjected to SDS-PAGE. Ab32067 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma using ab32067 at 1/50 dilution.
    • Anti-pro Caspase 7 antibody [Y33] (ab32067) at 1/1000 dilution + Jurkat cell lysate

      Predicted band size : 34 kDa
      Observed band size : 34 kDa

    References for Anti-pro Caspase 7 antibody [Y33] (ab32067)

    ab32067 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab32067.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up