Validated using a knockout cell line
Recombinant
RabMAb

Anti-pro Caspase 7 antibody [Y33] (ab32067)

Overview

  • Product name
    Anti-pro Caspase 7 antibody [Y33]
  • Description
    Rabbit monoclonal [Y33] to pro Caspase 7
  • Specificity
    The antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to N-terminal residues of human Pro-Caspase-7.

  • Positive control
    • Jurkat cell lysate and human lung squamous carcinoma.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/50.
ICC/IF 1/50.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
      -Gly-217' bond. Overexpression promotes programmed cell death.
    • Tissue specificity
      Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
    • Sequence similarities
      Belongs to the peptidase C14A family.
    • Post-translational
      modifications
      Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • apoptosis-related cysteine peptidase antibody
      • Apoptotic protease Mch-3 antibody
      • CASP-7 antibody
      • CASP7 antibody
      • CASP7_HUMAN antibody
      • Caspase-7 subunit p11 antibody
      • CMH-1 antibody
      • ICE-LAP3 antibody
      • ICE-like apoptotic protease 3 antibody
      see all

    Images



    • Predicted band size : 34 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Staurosporin treated HAP1 whole cell lysate (20 µg)
      Lane 3: CASP7 knockout HAP1 whole cell lysate (20 µg)
      Lane 4: Staurosporin treated CASP7 knockout HAP1 whole cell lysate (20 µg)
      Lane 5: HeLa whole cell lysate (20 µg)
      Lane 6: Staurosporin treated HeLa whole cell lysate (20 µg)

      Lanes 1 - 6: Merged signal (red and green). Green - ab32067 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab32067 was shown to recognize pro Caspase 7 when HAP1 + pro Caspase 7 knockout samples were used, along with additional cross-reactive bands. Wild-type and pro Caspase 7 knockout samples were subjected to SDS-PAGE. Ab32067 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma using ab32067 at 1/50 dilution.
    • Anti-pro Caspase 7 antibody [Y33] (ab32067) at 1/1000 dilution + Jurkat cell lysate

      Predicted band size : 34 kDa
      Observed band size : 34 kDa

    References

    ab32067 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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