Overview

  • Product nameAnti-pro Caspase 9 antibody [E84]
    See all pro Caspase 9 primary antibodies
  • Description
    Rabbit monoclonal [E84] to pro Caspase 9
  • SpecificityThis antibody only recognises the pro form of Caspase 9 (46kDa)
  • Tested applicationsSuitable for: WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near N-terminus of human Caspase-9

  • Positive control
    • Jurkat cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberE84
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab32068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/25.
  • Application notesIs unsuitable for ICC or IP.
  • Target

    • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
      Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
    • Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
    • Sequence similaritiesBelongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
    • Post-translational
      modifications
      Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
      Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation.
    • Information by UniProt
    • Database links
    • Alternative names
      • APAF-3 antibody
      • APAF3 antibody
      • Apoptotic protease Mch-6 antibody
      • Apoptotic protease-activating factor 3 antibody
      • CASP-9 antibody
      • CASP9 antibody
      • CASP9_HUMAN antibody
      • Caspase-9 subunit p10 antibody
      • ICE-LAP6 antibody
      • ICE-like apoptotic protease 6 antibody
      • ICELAP6 antibody
      • MCH6 antibody
      see all

    Anti-pro Caspase 9 antibody [E84] images

    • Anti-pro Caspase 9 antibody [E84] (ab32068) at 1/2000 dilution + Jurkat cell lysate at 10 µg

      Predicted band size : 46 kDa
      Observed band size : 46 kDa
    • Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Pro-Caspase-9 RabMAb (ab32068).

    References for Anti-pro Caspase 9 antibody [E84] (ab32068)

    This product has been referenced in:
    • Catuogno S  et al. miR-34c may protect lung cancer cells from paclitaxel-induced apoptosis. Oncogene : (2012). WB ; Human . Read more (PubMed: 22370637) »
    • Hou W  et al. Autophagic degradation of active caspase-8: a crosstalk mechanism between autophagy and apoptosis. Autophagy 6:891-900 (2010). WB . Read more (PubMed: 20724831) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab32068.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"