proMMP9 Mouse ELISA Kit (ab100732)
- Product nameproMMP9 Mouse ELISA KitSee all MMP9 kits ...
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 50 pg/ml
- Range74.07 pg/ml - 18000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 109.7 96% - 118% Serum 114.4 97% - 132% Plasma 112 101% - 121%
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Mouse
- Product overview
ab100732 Mouse proMMP9 (matrix metalloproteinase-9) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse proMMP9 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse proMMP9 coated on a 96-well plate. Standards and samples are pipetted into the wells and proMMP9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse proMMP9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of proMMP9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent 5x concentrated buffer. ForStandard/Sample (serum/plasma samples/cell culture medium ) diluent. 1 x 15ml Detection Antibody proMMP9: biotinylated anti-mouse proMMP9 2 vials proMMP9 Microplate (12 strips x 8 wells) coated with anti-mouse proMMP9 1 unit proMMP9 Standard Mouse (recombinant) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Defects in MMP9 are the cause of metaphyseal anadysplasia type 2 (MANDP2) [MIM:613073]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.
modificationsProcessing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated.
- 82 kDa matrix metalloproteinase-992 kDa gelatinase92 kDa type IV collagenase
- CLG4BCollagenase Type 4 beta Collagenase type IV 92 KD EC 220.127.116.11Gelatinase 92 KD Gelatinase BGelatinase beta GelatinaseBGELBMacrophage gelatinaseMANDP2Matrix metallopeptidase 9 (gelatinase B 92kDa gelatinase 92kDa type IV collangenase)Matrix metalloproteinase 9MMP 9MMP-9MMP9MMP9_HUMANType V collagenase
Our Abpromise guarantee covers the use of ab100732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for proMMP9 Mouse ELISA Kit (ab100732)
ab100732 has not yet been referenced specifically in any publications.