The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).
Use a concentration of 0.01 - 0.1 µg/ml.
Use a concentration of 2 - 5 µg/ml.
FunctionHormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
Involvement in diseaseAtrial standstill 2 Atrial fibrillation, familial, 6
Sequence similaritiesBelongs to the natriuretic peptide family.
Post-translational modificationsCleaved by CORIN upon secretion to produce the functional hormone. Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.