Recombinant
RabMAb

Anti-Proapoptotic Caspase Adaptor Protein antibody [EPR11544] (ab181205)

Overview

  • Product name
    Anti-Proapoptotic Caspase Adaptor Protein antibody [EPR11544]
    See all Proapoptotic Caspase Adaptor Protein primary antibodies
  • Description
    Rabbit Monoclonal [EPR11544] to Proapoptotic Caspase Adaptor Protein
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Proapoptotic Caspase Adaptor Protein aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8WU39

  • Positive control
    • Raji, Ramos and Molt-4 cell lysates; Human kidney and pancreas tissue; Ramos cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/100 - 1/250.
Flow Cyt 1/210.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    Proapoptotic Caspase Adaptor Protein associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. May exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity By similarity. Isoform 2 may be involved in regulation of apoptosis.
  • Cellular localization
    Isoform 1: Endoplasmic reticulum Isoform 2: Cytoplasm. Note: Diffuse granular localization in the cytoplasm surrounding the nucleus.
  • Database links
  • Alternative names
    • MZB1 antibody
    • Caspase 2 binding protein antibody
    • FLJ32987 antibody
    • HSPC190 antibody
    • Marginal zone B and B1 cell specific protein antibody
    • MEDA 7 antibody
    • Mesenteric estrogen dependent adipose 7 antibody
    • Mesenteric oestrogen dependent adipose gene 7 antibody
    • MGC29506 antibody
    • PACAP antibody
    • pERp1 antibody
    • Plasma cell-induced ER protein 1 antibody
    • Plasma cell-induced resident endoplasmic reticulum protein antibody
    • Plasma cell-induced resident ER protein antibody
    • Proapoptotic caspase adapter protein antibody
    see all

Images

  • All lanes : Anti-Proapoptotic Caspase Adaptor Protein antibody [EPR11544] (ab181205) at 1/50000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Ramos cell lysate
    Lane 3 : Molt-4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Proapoptotic Caspase Adaptor Protein with ab181205 at 1/250 dilution. The slide is counterstained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Proapoptotic Caspase Adaptor Protein with ab181205 at 1/250 dilution. The slide is counterstained with Hematoxylin.

  • Flow cytometric analysis of paraformaldehyde-fixed Ramos cells labeling Proapoptotic Caspase Adaptor Protein with ab181205 at 1/210 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.

References

ab181205 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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