Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Proapoptotic Caspase Adaptor Protein aa 100 to the C-terminus. The exact sequence is proprietary. Database link: Q8WU39
Raji, Ramos and Molt-4 cell lysates; Human kidney and pancreas tissue; Ramos cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).
1/100 - 1/250.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Proapoptotic Caspase Adaptor Protein associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. May exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity By similarity. Isoform 2 may be involved in regulation of apoptosis.
Isoform 1: Endoplasmic reticulum
Isoform 2: Cytoplasm. Note: Diffuse granular localization in the cytoplasm surrounding the nucleus.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Proapoptotic Caspase Adaptor Protein with ab181205 at 1/250 dilution. The slide is counterstained with Hematoxylin.
Flow Cytometry - Anti-Proapoptotic Caspase Adaptor Protein [EPR11544] antibody (ab181205)
Flow cytometric analysis of paraformaldehyde-fixed Ramos cells labeling Proapoptotic Caspase Adaptor Protein with ab181205 at 1/210 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.