Overview

  • Product name
    Anti-proBNP antibody [15F11]
    See all proBNP primary antibodies
  • Description
    Mouse monoclonal [15F11] to proBNP
  • Tested applications
    Suitable for: ELISA, WB, IP, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    ETSGLQEQRNHLQGK

    , corresponding to amino acids 13 - 27 of Human proBNP.

  • General notes
    Immunoglobulin concentrations vary from batch to batch. Please contact technical services for the latest information.

Properties

Applications

Our Abpromise guarantee covers the use of ab13115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 13 kDa.
IP Use at an assay dependent concentration. PubMed: 21482747
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [24E11] to proBNP (HRP) (ab13123). Can be used as capture antibody in conjunction with recommended pair. Recognises the antigen in blood of patients with heart faliure, unstable angina and myocardial infarction.

Target

  • Function
    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • Tissue specificity
    Brain and also in atria, but at much lower levels than ANP.
  • Sequence similarities
    Belongs to the natriuretic peptide family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANFB_HUMAN antibody
    • BNP antibody
    • BNP(1-32) antibody
    • BNP(5-29) antibody
    • BNP-32 antibody
    • Brain type natriuretic peptide antibody
    • Gamma brain natriuretic peptide antibody
    • Gamma-brain natriuretic peptide antibody
    • Natriuretic peptide precursor B antibody
    • nppb antibody
    • NT proBNP antibody
    see all

Images

  • NT-proBNP calibration curves using pairs ab14703 [10H4] and ab13111 [5B6] (grey), and ab13115 [15F11] and ab13111 [5B6] (black), (capture: 2 µg/well, detection: Eu-labeled 5B6; 200 ng/well). Antigen was recombinant NT-proBNP. Incubated 1 hr at room temperature
  • WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11]. WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11].
  • proBNP was immunoprecipitated from 7x106 HCT116 (human colon carcinoma cell line) cells with ab26 at 1/150 dilution. Western blot was performed from the immunoprecipitate using anti-p53 antibody. Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (ab175739) was used as secondary antibody at 1/5000 dilution.


    Lane 1: HCT116 whole cell lysate 10 µg (Input).

    Lane 2: ab207799 IP inetoposide treated HCT116 whole cell lysate.

    Lane 3: ab207799 IP inetoposide treated HCT116 p53-/- whole cell lysate (negative control).

References

This product has been referenced in:
  • Tonne JM  et al. Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes. Clin Chem 57:864-73 (2011). IP ; Human . Read more (PubMed: 21482747) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you very much for your inquiry.

I have to confirm that do not have any pro-BNP antibodies that are tested and guaranteed for rat.

I checked the immunogens for the available pro-BNP antibodies and unfortunately I cannot recomme...

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