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Our Abpromise guarantee covers the use of ab13115 in the following tested applications.
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Detects a band of approximately 13 kDa.|
|IP||Use at an assay dependent concentration. PubMed: 21482747|
|Sandwich ELISA||Use at an assay dependent dilution. Can be used as capture antibody in conjunction with recommended pair. Recognises the antigen in blood of patients with heart faliure, unstable angina and myocardial infarction.|
proBNP was immunoprecipitated from 7x106 HCT116 (human colon carcinoma cell line) cells with ab26 at 1/150 dilution. Western blot was performed from the immunoprecipitate using anti-p53 antibody. Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (ab175739) was used as secondary antibody at 1/5000 dilution.
Lane 1: HCT116 whole cell lysate 10 µg (Input).
Lane 2: ab207799 IP inetoposide treated HCT116 whole cell lysate.
Lane 3: ab207799 IP inetoposide treated HCT116 p53-/- whole cell lysate (negative control).