Overview

  • Product name
    Anti-proBNP antibody [5B6]
    See all proBNP primary antibodies
  • Description
    Mouse monoclonal [5B6] to proBNP
  • Tested applications
    Suitable for: ELISA, WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    HPLGSPGSASDL

    , corresponding to amino acids 1 - 12 of Human proBNP.

  • General notes

    Immunoglobulin concentrations vary from batch to batch (generally 4-6 mg/ml for this clone). Please contact technical services for the latest information.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab32842 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab13111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 13 kDa.
Sandwich ELISA Use at an assay dependent concentration.

Target

  • Function
    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • Tissue specificity
    Brain and also in atria, but at much lower levels than ANP.
  • Sequence similarities
    Belongs to the natriuretic peptide family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANFB_HUMAN antibody
    • BNP antibody
    • BNP(1-32) antibody
    • BNP(5-29) antibody
    • BNP-32 antibody
    • Brain type natriuretic peptide antibody
    • Gamma brain natriuretic peptide antibody
    • Gamma-brain natriuretic peptide antibody
    • Natriuretic peptide precursor B antibody
    • nppb antibody
    • NT proBNP antibody
    see all

Images

  • NT-proBNP calibration curves using pairs ab14703 [10H4] and ab13111 [5B6] (grey), and ab13115 [15F11] and ab13111 [5B6] (black), (capture: 2 µg/well, detection: Eu-labeled 5B6; 200 ng/well). Antigen was recombinant NT-proBNP. Incubated 1 hr at room temperature
  • WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11]. WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11].

References

This product has been referenced in:
  • Carlson C  et al. Phenotypic Screening with Human iPS Cell-Derived Cardiomyocytes: HTS-Compatible Assays for Interrogating Cardiac Hypertrophy. J Biomol Screen N/A:N/A (2013). Read more (PubMed: 24071917) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you very much for your inquiry.

I have to confirm that do not have any pro-BNP antibodies that are tested and guaranteed for rat.

I checked the immunogens for the available pro-BNP antibodies and unfortunately I cannot recomme...

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Thank you very much for sharing your data with us regarding the use of ab13111 in ELISA. I would like to encourage you to submit an Abreview via the online datasheet of ab13111 (accessible via the link below) so that other users can benefit from your e...

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Thank you for your email. For ab13111, the concentration of the batch that is currently in stock is 4.7 mg/ml. For ab14699, the concentration of the batch that is currently in stock is 5.80 mg/ml. If you have any additional questions, please contac...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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