Overview

  • Product nameProcalcitonin Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    27.43 pg/ml - 20000 pg/ml
  • Recovery

    83 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100.5 80% - 111%
    Serum 79.14 70% - 91%
    Plasma 72.35 65% - 88%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Procalcitonin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Procalcitonin in serum, plasma, and cell culture supernatants. (Human Procalcitonin concentration is pretty low in normal serum/plasma, it may not be detected in this assay)

    This assay employs an antibody specific for Human Procalcitonin coated on a 96-well plate. Standards and samples are pipetted into the wells and Procalcitonin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Procalcitonin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Procalcitonin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Procalcitonin Human ELISA Kit images

  • Procalcitonin measured in biologicals showing quantity (pg) per mL of tested sample

  • Representative Standard Curve using ab100630.

  • Representative Standard Curve using ab100630.

Protocols

References for Procalcitonin Human ELISA Kit (ab100630)

ab100630 has not yet been referenced specifically in any publications.

Product Wall

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Please recommend the customer to mix few micro litres of TMB solution with HRP-streptavidin solution on a glass slide. The colour developed should be blue which will change to yellow when stop solution is added. Read More

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1A, For storage please refer to the protocol and store the individual as given in the booklet.

"ab100630 may be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombina...

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The 8 well strips provided with ab100630 are not designed for single wells to be broken off and used individually.

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I am sorry for the delay in getting back to you. We are not open on the weekends. I am now able to confirm that you should be able to isolate individual wells of this product. As discussed in my previous email, the...

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Thank you for contacting Abcam. My colleague xxxxx passed on your enquiry to the Scientific Support
team and I hope I can help you with your questions.

The microplate provided with the Procalcitonin Human ELISA Kit (ab100630) is composed o...

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Attached please find the QC of the standard for our most recent lot of kits. Regarding sensitivity, the minimum detectable dose of procalcitonin is typically less than 30 pg/ml.

...

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To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the de...

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The Procalcitonin Human ELISA Kit (ab100630) has to our knowledge not been compared to performing the Brahms method of detecting human procalcitonin. The kit should however match up well with most of the reported...

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I understand your reluctance to purchase a kit that might not detect macaque PCT. Unfortunately, we do not have any free kits, expired or otherwise.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"