Recombinant Human proCathepsin D purified from E.coli with His tag.
MCF7, HepG2 and SK-BR3 cell lysates.
This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR155210 will be from tissue culture supernatant.
Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 45 kDa.
Use at an assay dependent concentration.
Use at 1μg
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Involvement in disease
Defects in CTSD are the cause of neuronal ceroid lipofuscinosis type 10 (CLN10) [MIM:610127]; also known as neuronal ceroid lipofuscinosis due to cathepsin D deficiency. A form of neuronal ceroid lipofuscinosis with onset at birth or early childhood. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy.
Belongs to the peptidase A1 family.
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.