Anti-procollagen type IIA antibody [003-02] (ab17771)

Overview

  • Product nameAnti-procollagen type IIA antibody [003-02]
  • Description
    Mouse monoclonal [003-02] to procollagen type IIA
  • Tested applicationsIHC-P, IHC-Fr more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment - N terminal pro-peptide (amino acids 26-181) of human collagen type II.

  • EpitopeEpitope is located at the amino-terminal part of procollagen type IIA.

Properties

Applications

Our Abpromise guarantee covers the use of ab17771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent dilution.
  • Application notesIs unsuitable for ELISA.
  • Target

    • RelevanceCollagen type II is the most abundant protein of cartilage matrix. Two isoforms exist and collagen type IIA is involved in early development of cartilage in embryos. The collagen is produced from type II procollagen when two teloterminal propeptides are digested from the precursor and released into the circulation. Procollagen type II N-terminal propeptide is considered a marker of cartilage synthesis and the normal concentration in serum is approximately 30ng/mL.
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • Alpha 1 type II collagen antibody
      • chondrocalcin included antibody
      • col11a3 antibody
      • Collagen II alpha 1 polypeptide antibody
      • collagen of cartilage antibody
      • PIIANP antibody
      • Procollagen type II alpha 1 antibody
      see all

    Anti-procollagen type IIA antibody [003-02] images

    • ab17771, at a 1/1000 dilution, staining fetal bone anlage in the hand (around week 10) by IHC-P.
    • ab17771, at a 1/1000 dilution, staining specific tumour cells of biopsy from a chondroblastoma by IHC-P.  The antibody was detected with an HRP conjugated Goat anti-Mouse antibody.
    • ab17771 staining mouse eye tissue sections -- conjunctiva and limbus by IHC-P.  The tissue was fixed with paraformaldehyde and antigen retrieval was heat mediated using citrate buffer.  The blocking agent was 1% TSA at 25°C for 1 hour.  The primary antibody was incubated (undiluted) at 25°C for 15 hours.  A biotinylated anti-mouse was used as the secondary antibody.

      See Abreview

    References for Anti-procollagen type IIA antibody [003-02] (ab17771)

    ab17771 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - other (human fibroblasts, chondrocytes,iPSC)
    Loading amount 30 µg
    Specification human fibroblasts, chondrocytes,iPSC
    Gel Running Conditions Reduced Denaturing (4-12%)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 21 2013

    Thank you very much for your call today and for your interest in ab17771.

    To our knowledge, ab17771 has not been tested in Western blot. However by participating in our AbTrial program you can now use our products in an untested application o...

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    Thank you for contacting us. After performing a BLAST search on the immunogen sequence, I am pleased to report that there is 97% identity with dog.  Though we cannot guarantee the antibody to work on this species because we do not have any data sho...

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    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (eye--conjunctiva and limbus)
    Specification eye--conjunctiva and limbus
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization No
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 10 2007

    Thank you for your enquiry. Unfortunately ab17771 is the only procollagen type IIA antibody that we currently carry. If you have any additional questions, please contact us again.

    We currently have only ab17771 which recognises procollagen type IIA and cross reacts with human. A blast search revealed that there is 92% identity between the human and the rat protein (3.5% are conservative substitutions and 3.5% are insertions or ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"