Anti-procollagen type IIA antibody (ab17771)


  • Product nameAnti-procollagen type IIA antibody
  • Description
    Mouse monoclonal to procollagen type IIA
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment - N terminal pro-peptide (amino acids 26-181) of human collagen type II.

  • EpitopeEpitope is located at the amino-terminal part of procollagen type IIA.



Our Abpromise guarantee covers the use of ab17771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.


  • RelevanceCollagen type II is the most abundant protein of cartilage matrix. Two isoforms exist and collagen type IIA is involved in early development of cartilage in embryos. The collagen is produced from type II procollagen when two teloterminal propeptides are digested from the precursor and released into the circulation. Procollagen type II N-terminal propeptide is considered a marker of cartilage synthesis and the normal concentration in serum is approximately 30ng/mL.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Alpha 1 type II collagen antibody
    • Collagen II alpha 1 polypeptide antibody
    • Procollagen type II alpha 1 antibody

Anti-procollagen type IIA antibody images

  • ab17771, at a 1/1000 dilution, staining fetal bone anlage in the hand (around week 10) by IHC-P.
  • ab17771, at a 1/1000 dilution, staining specific tumour cells of biopsy from a chondroblastoma by IHC-P.  The antibody was detected with an HRP conjugated Goat anti-Mouse antibody.
  • ab17771 staining mouse eye tissue sections -- conjunctiva and limbus by IHC-P.  The tissue was fixed with paraformaldehyde and antigen retrieval was heat mediated using citrate buffer.  The blocking agent was 1% TSA at 25°C for 1 hour.  The primary antibody was incubated (undiluted) at 25°C for 15 hours.  A biotinylated anti-mouse was used as the secondary antibody.

    See Abreview

References for Anti-procollagen type IIA antibody (ab17771)

This product has been referenced in:
  • Olderøy MØ  et al. Biochemical and structural characterization of neocartilage formed by mesenchymal stem cells in alginate hydrogels. PLoS One 9:e91662 (2014). IHC-P ; Human . Read more (PubMed: 24626259) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (human fibroblasts, chondrocytes,iPSC)
Loading amount 30 µg
Specification human fibroblasts, chondrocytes,iPSC
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jan 21 2013

Thank you very much for your call today and for your interest in ab17771.

To our knowledge, ab17771 has not been tested in Western blot. However by participating in our AbTrial program you can now use our products in an untested application o...

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Thank you for contacting us. After performing a BLAST search on the immunogen sequence, I am pleased to report that there is 97% identity with dog.  Though we cannot guarantee the antibody to work on this species because we do not have any data sho...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (eye--conjunctiva and limbus)
Specification eye--conjunctiva and limbus
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 10 2007

Thank you for your enquiry. Unfortunately ab17771 is the only procollagen type IIA antibody that we currently carry. If you have any additional questions, please contact us again.

We currently have only ab17771 which recognises procollagen type IIA and cross reacts with human. A blast search revealed that there is 92% identity between the human and the rat protein (3.5% are conservative substitutions and 3.5% are insertions or ...

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