SpecificityThis antibody blocks specifically with leumorphin (rat only). Cells are found in the paraventricular and supraoptic nucleus and fibres in the lateral hypothalamus in rat brain of colchicine treated 4% FA perfused tissue.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/2000.
FunctionLeu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opiod activity, it is 700 times more potent than Leu-enkephalin. Leumorphin has a typical opiod activity and may have anti-apoptotic effect.
Involvement in diseaseDefects in PDYN are the cause of spinocerebellar ataxia type 23 (SCA23) [MIM:610245]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA23 is an adult-onset autosomal dominant form characterized by slowly progressive gait and limb ataxia, with variable additional features, including peripheral neuropathy and dysarthria.
Sequence similaritiesBelongs to the opioid neuropeptide precursor family.
Post-translational modificationsThe N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Immunohistochemistry - Free Floating - Anti-ProDynorphin antibody (ab11137)This image is courtesy of an Abreview submitted by Kouichi Nakamura.
ab11137 staining ProDynorphin in Rat brain tissue by Immunohistochemistry - Free floating sections. The tissue was fixed by transcardial perfusion with 4% paraformaldehyde, 0.1% glutaraldeyde in 0.1M phosphate buffer (pH 7.4). The brain was postfixed with 4% paraformaldehyde in 0.1 M phosphate buffer (pH7.4) overnight at 4°C and then cut into 50 µm-thick parasagittal sections with a vibrating microtome. The primary antibody was diluted 1/100 in and incubated with the sample for 16 hours at 20°C. A Cy3®-conjugated Donkey anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/500.