Overview

  • Product nameAnti-ProDynorphin antibody
    See all ProDynorphin primary antibodies
  • Description
    Rabbit polyclonal to ProDynorphin
  • SpecificityThis antibody blocks specifically with leumorphin (rat only). Cells are found in the paraventricular and supraoptic nucleus and fibres in the lateral hypothalamus in rat brain of colchicine treated 4% FA perfused tissue.
  • Tested applicationsSuitable for: IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    RSQENPNTYSEDLDV

    (Rat)

  • General notesThis product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS
    Whole serum is supplied - diluted.
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200 - 1/2000.

Target

  • FunctionLeu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.
    Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opiod activity, it is 700 times more potent than Leu-enkephalin.
    Leumorphin has a typical opiod activity and may have anti-apoptotic effect.
  • Involvement in diseaseDefects in PDYN are the cause of spinocerebellar ataxia type 23 (SCA23) [MIM:610245]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA23 is an adult-onset autosomal dominant form characterized by slowly progressive gait and limb ataxia, with variable additional features, including peripheral neuropathy and dysarthria.
  • Sequence similaritiesBelongs to the opioid neuropeptide precursor family.
  • Post-translational
    modifications
    The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADCA antibody
    • Alpha neoendorphin antibody
    • Beta neoendorphin antibody
    • Beta-neoendorphin-dynorphin antibody
    • Big Dyn antibody
    • Big dynorphin antibody
    • Dyn antibody
    • Dyn-A17 antibody
    • Dyn-B antibody
    • Dynorphin A antibody
    • Dynorphin A(1-13) antibody
    • Dynorphin A(1-17) antibody
    • Dynorphin A(1-8) antibody
    • Dynorphin B antibody
    • Dynorphin B(1-13) antibody
    • Dynorphin B-29 antibody
    • Enkephalin B antibody
    • Leu enkephalin antibody
    • Leumorphin antibody
    • Neoendorphin dynorphin enkephalin prepropeptide antibody
    • PDYN antibody
    • PDYN_HUMAN antibody
    • PENKB antibody
    • Preprodynorphin antibody
    • Preproenkephalin B antibody
    • Prodynorphin antibody
    • Proenkephalin B antibody
    • Rimorphin antibody
    • SCA23 antibody
    see all

Anti-ProDynorphin antibody images

  • ab11137 staining ProDynorphin in Rat brain tissue by Immunohistochemistry - Free floating sections. The tissue was fixed by transcardial perfusion with 4% paraformaldehyde, 0.1% glutaraldeyde in 0.1M phosphate buffer (pH 7.4). The brain was postfixed with 4% paraformaldehyde in 0.1 M phosphate buffer (pH7.4) overnight at 4°C and then cut into 50 µm-thick parasagittal sections with a vibrating microtome. The primary antibody was diluted 1/100 in and incubated with the sample for 16 hours at 20°C. A Cy3®-conjugated Donkey anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/500.

    See Abreview

References for Anti-ProDynorphin antibody (ab11137)

This product has been referenced in:
  • Romero-Picó A  et al. Hypothalamic ?-opioid receptor modulates the orexigenic effect of ghrelin. Neuropsychopharmacology 38:1296-307 (2013). Read more (PubMed: 23348063) »
  • Schlosburg JE  et al. Long-term antagonism of ? opioid receptors prevents escalation of and increased motivation for heroin intake. J Neurosci 33:19384-92 (2013). Rat . Read more (PubMed: 24305833) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step None
Permeabilization Yes - 0.3% Triton X-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Formalin
Username

Miss. Allisa Song

Verified customer

Submitted Feb 26 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA+NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (Cortex)
Specification Cortex
Permeabilization Yes - 0.3% Triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 18 2014

Application Immunohistochemistry free floating
Sample Rat Tissue sections (Brain)
Specification Brain
Username

Dr. Kouichi Nakamura

Verified customer

Submitted Mar 24 2011

I have contacted our accounting department and you will be issued a refund for your order for ab11137. This process does take a couple of weeks and if you have any questions regarding the refund please email: accounts@abcam.com If you have any future ...

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Thank you for your patience and the details that you have provided us with. To our knowledge, this antibody has only been tested for application in IHC-frozen sections. We do not know how the antibody works in paraformaldehyde or formalin-fixed section...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"