Overview

  • Product nameAnti-Profilin 1 antibody [1D5]
    See all Profilin 1 primary antibodies
  • Description
    Mouse monoclonal [1D5] to Profilin 1
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant full length Human Profilin 1 produced in HEK293T cells (NP_005013).

  • Positive control
    • HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cell extracts; Human Kidney and pancreas tissues; HeLa and Jurkat cells; COS7 cells transiently transfected by pCMV6-ENTRY Profilin 1; HEK293T cell lysate transfected with pCMV6-ENTRY Profilin 1 cDNA; HEK293T cells transfected with pCMV6-ENTRY Profilin 1 overexpress plasmid.
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Applications

Our Abpromise guarantee covers the use of ab118984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 15 kDa.
IHC-P 1/50.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionBinds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
  • Sequence similaritiesBelongs to the profilin family.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin binding protein antibody
    • ALS18 antibody
    • Epididymis tissue protein Li 184a antibody
    • OTTHUMP00000125244 antibody
    • PFN 1 antibody
    • Pfn antibody
    • PFN1 antibody
    • PROF1_HUMAN antibody
    • Profilin I antibody
    • Profilin-1 antibody
    • Profilin1 antibody
    • ProfilinI antibody
    see all

Anti-Profilin 1 antibody [1D5] images

  • All lanes : Anti-Profilin 1 antibody [1D5] (ab118984) at 1/1000 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell lysate
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 15 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202338)
  • All lanes : Anti-Profilin 1 antibody [1D5] (ab118984) at 1/1000 dilution

    Lane 1 : HEK293T cell lysate transfected with the pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with the pCMV6-ENTRY Profilin 1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 15 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202338)
  • ab118984 at 1/50 dilution staining Profilin 1 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
  • ab118984 at 1/50 dilution staining Profilin 1 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.
  • ab118984 at 1/100 dilution staining Profilin 1 in COS7 cells transiently transfected with pCMV6-ENTRY Profilin 1 by Immunofluorescence.
  • ab118984 at 1/100 dilution staining Profilin 1 in HEK293T cells transfected with either pCMV6-ENTRY Profilin 1 overexpressing plasmid (Red) or empty vector control plasmid (Blue) by flow cytometry.
  • ab118984 at 1/100 dilution staining Profilin 1 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • ab118984 at 1/100 dilution staining Profilin 1 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

References for Anti-Profilin 1 antibody [1D5] (ab118984)

This product has been referenced in:
  • Ralhan R  et al. Immunohistochemical Subcellular Localization of Protein Biomarkers Distinguishes Benign from Malignant Thyroid Nodules: Potential for Fine-Needle Aspiration Biopsy Clinical Application. Thyroid 25:1224-34 (2015). IHC . Read more (PubMed: 26131603) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"