Overview

  • Product name
  • Description
    Rabbit polyclonal to Profilin 1
  • Specificity
    Sequence similarity is 70% similar to the corresponding sequence in Profilin 2.
  • Tested applications
    Suitable for: Indirect ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Pig
  • Immunogen

    Synthetic peptide:

    AGWNAYIDNLMADGTC

    conjugated to KLH, corresponding to N terminal amino acids 2-17 of Human Profilin 1

  • Positive control
    • Whole extracts of mouse NIH-3T3 cells, human HeLa cells (if applying chemiluminescent detection reagent) and rat NRK cells (via indirect immunofluorescence).

Properties

Applications

Our Abpromise guarantee covers the use of ab50668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use a concentration of 10 - 20 µg/ml. (recommended using rat NRK cells).
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). (using extracts of mouse NIH-3T3 and human HeLa cells, applying a chemiluminescent detection reagent).

Target

Images

  • All lanes : Anti-Profilin 1 antibody (ab50668) at 0.5 µg/ml

    Lane 1 : Whole Mouse 3T3 cell extract
    Lane 2 : Whole Mouse 3T3 cell extract with Profilin 1 (N-terminal) immunizing peptide

    Secondary
    Goat Anti-Rabbit IgG
    Developed using the ECL technique

    Predicted band size : 15 kDa
    Observed band size : 15 kDa

References

ab50668 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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