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Rabbit polyclonal to Profilin 1
Predicted to work with:
Mouse, Rat, Cow, Pig
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Profilin 1.
This antibody gave a positive signal in the following whole cell lysates: NIH3T3; HeLa; HEK293; HuES7; HepG2; Jurkat.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
Belongs to the profilin family.
Cytoplasm > cytoskeleton.
Information by UniProt
Actin binding protein antibody
Epididymis tissue protein Li 184a antibody
Western blot - Profilin 1 antibody (ab92803)
All lanes :
Anti-Profilin 1 antibody (ab92803) at 1 µg/ml
Lane 1 :
NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 :
HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 :
HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lane 5 :
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 :
Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"