Anti-Profilin 1 antibody - Carboxyterminal end (ab50667)

Overview

  • Product nameAnti-Profilin 1 antibody - Carboxyterminal end
    See all Profilin 1 primary antibodies
  • Description
    Rabbit polyclonal to Profilin 1 - Carboxyterminal end
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide:

    CYEMASHLRRSQY

    conjugated to KLH, corresponding to C terminal amino acids 128-140 of Human Profilin 1

  • Positive control
    • Immunoblotting: whole cell extract of human HeLa cells, Immunoprecipitation: Mouse NIH-3T3 cell lysates Indirect Immunofluorescence: rat NRK cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab50667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 - 20 µg/ml.
Flow Cyt 1/200. (see Abreview)



ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
IP Use at an assay dependent concentration.

Target

Anti-Profilin 1 antibody - Carboxyterminal end images

  • Lane 1: Positve control — Western blot: ab50667 at a concentration of 1μg/ml on whole extract of Mouse 3T3 cells at 10μg/ml.

    Lane 2: Immunoprecipitation — 10 μg of ab50667 was used to immunoprecipitate Mouse Profilin 1 from 3T3 cell lysate (20μg/ml). Detection antibody: Rabbit Anti-Profilin 1 (N-terminal) antibody.

    Lane 3: Negative control — non-relevant antibody was used for IP. Detection antibody: Rabbit Anti-Profilin 1 (N-terminal) antibody

  • ab50667 detecting Profilin 1 in Human platelets by Flow Cytometry. Platelets were isolated by spinning Platelet rich plasma on Histopaque. Cells were fixed in PFA and permeabilized in 0.1% Triton-X100 in 2% BSA for 30 minutes. The cell population was gated on platelets. The primary antibody was diluted 1/200 in 2% BSA in PBS and incubated with the sample for 18 hours at 4°C. An Alexa Fluor® 488-conjugated Chicken anti-Rabbit IgG (H+L) antibody, diluted 1/500 was used as the secondary antibody.

    Abbreviations in the figure:
    US Unstained, Red Peak;
    IgG Rb: IgG Rabbit (Isotype Control), Blue Peak;
    Profilin: Profilin 1, Green peak.

    See Abreview

  • Anti-Profilin 1 antibody - Carboxyterminal end (ab50667) at 1/1000 dilution + Human platelet lysate at 20 µg

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Image courtesy of Mahesh Shivananjappa, United States

    See Abreview

References for Anti-Profilin 1 antibody - Carboxyterminal end (ab50667)

This product has been referenced in:
  • Guo Z  et al. A Legionella effector modulates host cytoskeletal structure by inhibiting actin polymerization. Microbes Infect N/A:N/A (2013). Read more (PubMed: 24286927) »
  • Viitanen M  et al. Experimental studies of mitochondrial function in CADASIL vascular smooth muscle cells. Exp Cell Res : (2012). Read more (PubMed: 23036509) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (RAW264.7)
Specification RAW264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton + 2% BSA in PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Apr 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Platelets)
Loading amount 20 µg
Specification Platelets
Treatment ADP for 30 min
Gel Running Conditions Reduced Denaturing (4-20% Criterion 26 Well)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Nov 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Platelets)
Specification Platelets
Preparation Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy Platelets
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"